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We narrowed to 10,323 results for: Coli

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Showing: 61 - 90 of 10323 results

  4. pHcRed-NPM1cOligodN243-C1

    Plasmid
    #131847
    Purpose
    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection
    Depositor
    Mark Minden
    Article
    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:
    Insert
    NPM1 (NPM1 Human)
    Tags
    HcRed
    Expression
    Mammalian
    Mutation
    This is a deletion contruct of the cytoplasmic m…
    Available Since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    131847-plasmid-map-sequence-id-264401

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  5. pOpen-ECOligA

    Plasmid
    #165564
    Purpose
    Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    E. coli DNA Ligase
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165564-plasmid-map-sequence-id-340043

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  15. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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  18. E. coli Lig-N-HiBiT

    Plasmid
    #215437
    Purpose
    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215437-plasmid-map-sequence-id-428719

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  19. E. coli Lig-C-HiBiT

    Plasmid
    #215438
    Purpose
    Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215438-plasmid-map-sequence-id-428720

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  21. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  22. pOpen-Endonuclease VIII E. coli

    Plasmid
    #165561
    Purpose
    Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease VIII E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165561-plasmid-map-sequence-id-339954

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Showing: 61 - 90 of 10323 results