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Plasmid#180775PurposeThis vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.DepositorTypeEmpty backboneUseAAV, Cre/Lox, and RNAiExpressionMammalianAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only
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pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV Retrograde)
Viral Prep#44362-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo (AAV Retrograde)
Viral Prep#55637-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP Smad3
Plasmid#15723DepositorAvailable SinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRetroSuper-GFP Smad4
Plasmid#15724DepositorAvailable SinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV Retrograde)
Viral Prep#44361-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsSNAP-tag (SNAPf)ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSIH1-puro-control shRNA
Plasmid#26597PurposeLentiviral control shRNA vector with puromycin selection.DepositorTypeEmpty backboneUseLentiviral and RNAiAvailable SinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro Scr shRNA
Plasmid#30520DepositorInsertScr shRNA
UseRNAi and RetroviralExpressionMammalianAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG3/κ
Plasmid#61885PurposeExpresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his
Plasmid#24055DepositorUseBicistronicTagsMyc (hVps34) and V5/his (hVps15)ExpressionMammalianAvailable SinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry (AAV Retrograde)
Viral Prep#50459-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-pgk-Cre (AAV Retrograde)
Viral Prep#24593-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterpgkAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-M80-F2-IgG1/κ
Plasmid#50383PurposeExpresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chainDepositorInsertshuman Gamma 1 heavy chain expression cassette with unknown specificity
human Kappa light chain expression cassette with unknown specificity
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available SinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab1aWT
Plasmid#46776Purposeexpression of WT Rab1a in mammalian cellsDepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE zeo Ecotropic Receptor
Plasmid#10687DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgM/κ
Plasmid#61881PurposeExpresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)DepositorInsertsHER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2- Empty control -SV40-Luc
Plasmid#26280PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianAvailable SinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-Cox8A Control Plasmid
Plasmid#101129PurposeExpression of SNAP-Tagged Cox8A in Mammalian cellsDepositorAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGP-pcDNA3.1 Puro-CAG-Voltron2
Plasmid#172909PurposeMammalian expression of voltage sensorDepositorInsertVoltron2
ExpressionMammalianMutationA122DPromoterCAGAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Control-PTEN-3'UTR-Wt
Plasmid#21326DepositorAvailable SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149198)
Plasmid#80248Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Luc-pSUPER-Retro-GFP/Neo
Plasmid#22133DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…Available SinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP T2A Cre
Plasmid#63704PurposeTetracycline or doxycycline inducible expression of GFP T2A Cre fusion in mammalian cellsDepositorInsertT2A Cre
UseRetroviralTagsEGFPExpressionMammalianPromotertight TRE promoterAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-ADRβ2 Control Plasmid
Plasmid#101123PurposeExpression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cellsDepositorInsertBeta-2-Adrenergic Receptor (ADRB2 Human)
TagsSNAP-tag (SNAPf)ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)
Viral Prep#107738-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-gfap(Intron1/5'/Exon1-zebrafish)
Plasmid#39761DepositorAvailable SinceAug. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-control-APEX
Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorInsertcontrol-APEX (Nphp3 Glycine max, Mouse)
Tagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Flpo (AAV Retrograde)
Viral Prep#55634-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. Flpo and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV Retrograde)
Viral Prep#104488-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV Retrograde)
Viral Prep#87306-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV Retrograde)
Viral Prep#20298-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro Scr shRNA
Plasmid#30520DepositorInsertScr shRNA
UseRNAi and RetroviralExpressionMammalianAvailable SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry (AAV Retrograde)
Viral Prep#50474-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his
Plasmid#24055DepositorUseBicistronicTagsMyc (hVps34) and V5/his (hVps15)ExpressionMammalianAvailable SinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only