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Showing: 101 - 150 of 11158 results

  2. Human Genome-Wide Reduced Double-gRNA library

    Pooled Library
    #137999
    Purpose
    This gRNA library targets the human coding genome, aimed to reduce the scale of genome-wide perturbation screens up to fivefold compared to conventional methods, without sacrificing performance.
    Depositor
    Leopold Parts
    Article
    Peets et al bioRxiv 859652
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  7. Pan-Druggable Cancer Library

    Pooled Library
    #182133
    Purpose
    All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.
    Depositor
    Prashant Mali
    Article
    Hu et al Biomaterials. 2022 Jan;280:121276. doi:
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    Aug. 26, 2022
    Availability
    Academic Institutions and Nonprofits only

  9. MK01

    Bacterial Strain
    #195090
    Purpose
    Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).
    Depositor
    Sander Tans
    Article
    Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1
    Bacterial Resistance
    Chloramphenicol
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. MG1655-tMMR

    Bacterial Strain
    #62392
    Purpose
    E. coli MG1655 temperature-sensitive mismatch repair deficient strain, harboring mutS(A134V) mutL(G62S), and nadA::Tn10 pglΔ8 [λ cI857 Δ(cro-bioA)]} for λ Red expression
    Depositor
    Csaba Pál
    Article
    Nyerges et al Nucleic Acids Res. 2014 Apr;42(8):e62. d
    Bacterial Resistance
    Tetracycline
    Species
    Escherichia coli
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pRDA_936

    Plasmid
    #216094
    Purpose
    Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD46, CD47, CD55, CD81 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216094-plasmid-map-sequence-id-431378

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  20. AA054

    Plasmid
    #215932
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215932-plasmid-map-sequence-id-431474

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  21. AA016

    Plasmid
    #215927
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215927-plasmid-map-sequence-id-431438

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  22. AA102

    Plasmid
    #215935
    Purpose
    Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215935-plasmid-map-sequence-id-431435

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  23. AA107

    Plasmid
    #215936
    Purpose
    Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215936-plasmid-map-sequence-id-431416

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  25. pRDB_131

    Plasmid
    #216095
    Purpose
    Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD97, CD4, CD26, CD274 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216095-plasmid-map-sequence-id-431381

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  30. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  33. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

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  34. pET24a MDHNP_ARATH

    Plasmid
    #204238
    Purpose
    Bacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHNP_ARATH (AT5G58330 Mustard Weed)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include the chloroplastic transit sequen…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204238-plasmid-map-sequence-id-413341

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  36. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Use
    Tags
    Met-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

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  37. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204230-plasmid-map-sequence-id-413333

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  41. pSMART-ala10

    Plasmid
    #87135
    Purpose
    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Article
    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.
    Inserts
    NADPH Alanine Dehydrogenase (ald Synthetic)
    alanine exporter (alaE Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D196A, L197R
    Promoter
    waaHp from E. coli as part of operon with ald* an…
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87135-plasmid-map-sequence-id-174451

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  45. ∆cosσε ∆HG

    Bacterial Strain
    #197132
    Purpose
    Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.
    Depositor
    Baojun Wang
    Article
    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60
    Bacterial Resistance
    Hygromycin
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only

  46. V. natriegens NC7

    Bacterial Strain
    #215356
    Purpose
    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.
    Depositor
    Buz Barstow
    Bacterial Resistance
    None
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only

  47. V. natriegens NC1

    Bacterial Strain
    #215355
    Purpose
    Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.
    Depositor
    Buz Barstow
    Bacterial Resistance
    None
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only

  48. pET24a MDH_AQUAR

    Plasmid
    #204239
    Purpose
    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_AQUAR
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204239-plasmid-map-sequence-id-413339

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  49. pET24a MDH_9CREN

    Plasmid
    #204243
    Purpose
    Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_9CREN
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204243-plasmid-map-sequence-id-413343

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  50. pJK369

    Plasmid
    #73594
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    FLAG tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73594-plasmid-map-sequence-id-136122

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Showing: 101 - 150 of 11158 results