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pEM705 Gst (Ser)

Plasmid

#109354

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine at position 391 to Serine (Pertu…

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEM705 Gso (Ser)

Plasmid

#109353

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine at position 391 to Serine (Pertu…

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAZ_14-3-3

Plasmid

#109808

Purpose

Protein expression and purification of YWHAZ_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAZ_14-3-3 (YWHAZ Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Dvl2-GFP

Plasmid

#109148

Purpose

Stable Dvl2 expression

Depositor

Mariann Bienz

Article

Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

Insert

Dvl2 (DVL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHS18 (mANGPTL3-Strep)

Plasmid

#109111

Purpose

Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL3 (Angptl3 Mouse)

Tags

StrepII

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-TRAIL

Plasmid

#10953

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pWL1 (mANGPTL8-V5-His)

Plasmid

#109113

Purpose

Expresses V5 and His-tagged mouse ANGPTL8 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (Angptl8 Mouse)

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-X1-Neo-GFP-FAM134B

Plasmid

#109026

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-FAM134B-LIRmut

Plasmid

#109027

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B-LIRmut (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

Promoter

EIF-1a promoter

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shFAM134B

Plasmid

#109013

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

shFAM134B (RETREG1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDS-Red-N1 GABAA beta3

Plasmid

#109143

Purpose

The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.

Depositor

Stefano Vicini

Insert

GABAa receptor

Tags

RFP

Expression

Mammalian

Mutation

beta3 subunit of the GABAa receptor

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-CtIP

Plasmid

#109403

Purpose

Expresses Cas9 fusion to human CtIP protein

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

CtIP (RBBP8 Human)

Mutation

full length CtIP

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAE_14-3-3

Plasmid

#109942

Purpose

Protein expression and purification of YWHAE_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAE_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SFN_14-3-3

Plasmid

#109937

Purpose

Protein expression and purification of SFN_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SFN_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pD864-LacZ

Plasmid

#109380

Purpose

Rhamnose inducible LacZ expression. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pRHAM-LacZ

Expression

Bacterial

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCas9-Geminin

Plasmid

#109401

Purpose

Expresses Cas9 fusion to Geminin degron domain

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

Geminin (GMNN Human)

Mutation

Geminin degron fragment

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKM492-Myc-His

Plasmid

#109283

Purpose

ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Myc-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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