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pB-MultiplexedReporter-MOR170-1

Plasmid

#129461

Purpose

piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

MOR170-1 (Olfr895 Mouse)

Use

Synthetic Biology

Tags

LUCY tag, Rho tag

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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ROSA26(MultiFPsΔPuro)

Plasmid

#140759

Purpose

Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Inserts

puromycin-N-acetyltransferase
Venus
mCherry
mCerulean

Use

Mouse Targeting

Promoter

CAGGS

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hB

Plasmid

#60992

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EBFP2

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hY

Plasmid

#60995

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-B

Plasmid

#60978

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EBFP2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EBFP2

Use

Zebrafish

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mG

Plasmid

#60987

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EGFP

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EGFP

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hR

Plasmid

#60997

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mR

Plasmid

#60990

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hG

Plasmid

#60994

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear EGFP

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EGFP

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hC

Plasmid

#60993

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear cerulean

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

Cerulean

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mB

Plasmid

#60985

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EBFP2

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mO

Plasmid

#60989

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKO

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKO

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-mY

Plasmid

#60988

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-hO

Plasmid

#60996

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear mKO

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKO

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mC

Plasmid

#60986

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound cerulean

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

Cerulean

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-R

Plasmid

#60983

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Promoter

Bactin2

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-Y

Plasmid

#60981

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic citrine

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

citrine

Use

Zebrafish

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-fR

Plasmid

#60984

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKate2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKate2

Use

Zebrafish

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-G

Plasmid

#60980

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EGFP

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EGFP

Use

Zebrafish

Promoter

Bactin2

Available Since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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