We narrowed to 56,262 results for: plasmid

1. PAX6 donor plasmid

   Plasmid

   #105239

   Purpose

   Knock in H2B-GFP into the human PAX6 locus

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

   Insert

   PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
   

   Use

   TALEN

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Spacer Switch Plasmid

   Plasmid

   #215346

   Purpose

   Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

   Depositor

   Hank Seifert

   Article

   Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

   Insert

   OpaD spacer; Chloramphenicol Resistance Cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. "AND-gate" receiver plasmid

   Plasmid

   #127676

   Purpose

   Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

   Insert

   LuxR, GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLlacO-1 for LuxR, pLux for GFP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH29_32 series plasmid

   Plasmid

   #120901

   Purpose

   Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   Ec-TTL-P109
   sRNA-A targeting sequence
   RBS TIR=974 of GFPop1 gene
   GFPop1
   RBS TIR=6474 of ECF32mut gene
   ECF32mut
   T7 terminator
   resource competitor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BGGLO QPCR PLASMID

   Plasmid

   #79905

   Purpose

   Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

   Depositor

   Jeffery Miller

   Article

   Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

   Insert

   Human partial HBG and HBB gene
   

   Promoter

   No promoter

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. BRD4 CRISPRi plasmid

   Plasmid

   #154890

   Purpose

   Human BRD4 CRISPRi gRNA

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   BRD4-targeting gRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB1C3_YPet-PCP (y-plasmid)

   Plasmid

   #127637

   Purpose

   Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   YPet
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PP7 coat protein (PCP)

   Expression

   Bacterial

   Promoter

   pJ21306

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-Puro-U6-gRNA-pA plasmid

   Plasmid

   #247671

   Purpose

   spCas9 gRNA is driven by human U6 promoter, with the gRNA cassette between PuroR and pA, making it detectable in polyA-based RNA-seq.

   Depositor

   Tae Hyun Kim

   Insert

   Puromycin resistant gene
   

   Expression

   Mammalian

   Promoter

   TK promoter

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCT-Universal plasmid

   Plasmid

   #221852

   Purpose

   A universal plasmid for expressing any protein with TetraCysteine motif

   Depositor

   Artur Krężel

   Article

   Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

   Insert

   Tetra Cysteine tag
   

   Expression

   Bacterial

   Mutation

   No

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid

    #181748

    Purpose

    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Type

    Empty backbone

    Use

    Substrate plasmid

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Negative feedback (NF) plasmid

    Plasmid

    #169747

    Purpose

    Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-tetO2

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB1C3_mTq-Tat (c-plasmid)

    Plasmid

    #127635

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PCP and RNA binding peptide:…

    Expression

    Bacterial

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Plasmid L5 attB::Pleft* mScarlet

    Plasmid

    #169410

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mScarlet proper
    

    Use

    Unspecified

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSNAPf-Cox8A Control Plasmid

    Plasmid

    #101129

    Purpose

    Expression of SNAP-Tagged Cox8A in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Cox8A (COX8A Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pSNAPf-H2B Control Plasmid

    Plasmid

    #101124

    Purpose

    Expression of SNAP-tagged Histone H2B in mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. RNA motif plasmid EDEN15

    Plasmid

    #107252

    Purpose

    EDEN15 RNA motif

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    EDEN15 motif
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSNAPf-ADRβ2 Control Plasmid

    Plasmid

    #101123

    Purpose

    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pSNAP-CaaX Control Plasmid

    Plasmid

    #101133

    Purpose

    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    CaaX Box
    

    Tags

    SNAP-tag (SNAP26m)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Plasmid L5 attB::Pleft* mWasabi

    Plasmid

    #169409

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mWasabi
    

    Use

    Unspecified

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV sgRNA Expression Plasmid

    Plasmid

    #174540

    Purpose

    Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

    Depositor

    Ormond MacDougald

    Article

    Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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