We narrowed to 17,951 results for: puro
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Plasmid#164124PurposeBackbone for lentiviral stgRNA library construction (library 1 and 2)DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhuman U6Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro
Plasmid#89994PurposeDonor template for generation of NGN3-ntdTomato reporter cell linesDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro
Plasmid#121175PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell linesDepositorInsertGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Puro
Plasmid#211364PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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PB_iNEUROD1_P2A_GFP_Puro
Plasmid#168803PurposePiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expressionDepositorInsertNEUROD1 (NEUROD1 Synthetic, Human)
UseSynthetic Biology; PiggybacExpressionMammalianPromoterTREAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.puro_shGLS
Plasmid#110335PurposeshGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selectionDepositorInsertGLS glutaminase (GLS Synthetic, Human)
UseLentiviral and RNAiExpressionMammalianPromoterU6 (RNA PolIII)Available SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_pBabePuro
Plasmid#54337PurposeN-terminally FLAG tagged human IRE1a in retroviral pBabe puro vectorDepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationThe endogenous IRE1a signal sequence (aa1-18) was…Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_deltaP29_D408_pBabePuro
Plasmid#58422Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids P29 to D408Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ubc.Luc.IRES.Puro
Plasmid#33307DepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseExpressionMammalianPromoterUbcAvailable SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EGFP_Donor
Plasmid#178088PurposeHDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEGFP
UseCRISPRExpressionMammalianPromoterHuman PGK1Available SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE_puro
Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralPromoterCMVAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
ExpressionMammalianPromoterCMVAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnExpressionMammalianPromoterTet-On 3G BidirectionalAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianPromoterhuman PGK1Available SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Synthetic, Human)
UseSynthetic Biology; PiggybacExpressionMammalianPromoterTREAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Puro
Plasmid#125594PurposeLentiviral expression of sgRNA with GFP and puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 promoter for sgRNA expression and EFS promoter…Available SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only