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Showing: 1451 - 1500 of 2374 results

  6. pCXLE-dCas9VPPH-T2A-GFP-shP53

    Plasmid
    #102897
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPPH-T2A-GFP-shP53
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102897-plasmid-map-sequence-id-194735

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  9. pcDNA_DiCre_60.F2

    Plasmid
    #60208
    Purpose
    Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)
    Depositor
    Jean-Paul Herman
    Article
    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13
    Insert
    Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
    Use
    Tags
    FRAP and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    amino acids 2021-2112 of FRAP with a T2098L mutat…
    Promoter
    CMV
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    60208-plasmid-map-sequence-id-96298

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  12. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199368
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOstar-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199368-plasmid-map-sequence-id-389900

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  15. pCXLE-dCas9VPP300-T2A-GFP-shP53

    Plasmid
    #102896
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    dCas9VPP300-T2A-GFP-shP53
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102896-plasmid-map-sequence-id-194734

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  16. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid
    #163930
    Purpose
    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163930-plasmid-map-sequence-id-323460

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  23. pCY5.1nh

    Plasmid
    #160299
    Purpose
    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160299-plasmid-map-sequence-id-464600

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  24. pCY5.1kn

    Plasmid
    #160298
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160298-plasmid-map-sequence-id-464598

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  26. Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

    Plasmid
    #216403
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Alanine 51, 56 and Glutamine 70, 443 to P…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216403-plasmid-map-sequence-id-430104

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  27. Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

    Plasmid
    #216404
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Alanine 51, 56, Glutamine 70, 443 and Asp…
    Promoter
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216404-plasmid-map-sequence-id-430105

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Showing: 1451 - 1500 of 2374 results