We narrowed to 727 results for: ELP

1. M13cp-dg3 hp

   Plasmid

   #218095

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRC-CMV-Rev1b

   Plasmid

   #204153

   Purpose

   Lentivirus helper plasmid coding for Rev

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   Rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNHEJ-Cas12a-recX

   Plasmid

   #158714

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pmyc

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNHEJ-Cas9-recX

   Plasmid

   #158715

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNP2270

   Plasmid

   #222733

   Purpose

   pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (lacZ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. M13cp-dg2 hp

   Plasmid

   #218094

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pII

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN716 - pCAGGS-eGFP-3Xnls-bpA

   Plasmid

   #156435

   Purpose

   For transient expression of nuclear eGFP in mammalian cells

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. M13cp-dg1 hp

   Plasmid

   #218093

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSH1/Mf-del-Akt-E

    Plasmid

    #15288

    Depositor

    David Spencer

    Article

    Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

    Insert

    delPH-Akt (Akt1 Mouse)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain Fy…

    Expression

    Mammalian

    Mutation

    Truncated PH (residues 1-99)

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Use

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSSA-DsRed/eGFP (pXK-442)

    Plasmid

    #208827

    Purpose

    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHDR-PMG (pXK-1650)

    Plasmid

    #208858

    Purpose

    HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSSA-eGFP (pXK-221)

    Plasmid

    #208826

    Purpose

    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

    Plasmid

    #156436

    Purpose

    For transient expression of human PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5B (PDS5B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

    Plasmid

    #156440

    Purpose

    For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA1 (Stag1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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