We narrowed to 703 results for: ELP
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Plasmid#208827PurposeThe SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pHDR-PMG (pXK-1650)
Plasmid#208858PurposeHDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA
Plasmid#156445PurposeFor transient expression of mouse RAD21 (SCC1) tagged with mKate2DepositorInsertmouse RAD21 (Rad21 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorInserthuman PDS5B (PDS5B Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA
Plasmid#156440PurposeFor transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2DepositorInsertmouse SA1 (Stag1 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW17 - pAdapter
Plasmid#174664PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.DepositorInsertMS2 coat protein
UseTagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1082_Rad21_STOP
Plasmid#156450PurposeFor transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codonDepositorInsertCas9, RAD21 (Rad21 Mouse)
UseCRISPRTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA
Plasmid#156439PurposeFor transient expression of mouse PDS5A tagged with mKate2DepositorInsertmouse PDS5A (Pds5a Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p35u4
Plasmid#174786PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid systemDepositorInsertMS2 coat protein
UseTagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHDR-eGFP (pXK-1647)
Plasmid#208824PurposeThe HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDRDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA
Plasmid#156441PurposeFor transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2DepositorInsertmouse SA2 (Stag2 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-MFA-NFAPoptim
Plasmid#221090PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt
Plasmid#175563PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1DepositorInsertGOXL1
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG3H-PE3max-attR1R2
Plasmid#213049PurposeDestination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sitesDepositorInsertsCas9 nickase
M-MLV Reverse Transcriptase
UseNote: this plasmid needs helper pvs1-vir2 for rep…TagsExpressionPlantMutationH840A, R240K, N413K,PromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only