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pVAX-Help-SIINFEKL

Plasmid

#141350

Purpose

DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

HELP-SIINFEKL-KDEL

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCaSper4 cyto roGFP2-Orp1

Plasmid

#64998

Purpose

Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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p3003 pGEM E6-AP

Plasmid

#8644

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pB194

Plasmid

#61154

Purpose

Expression of KIN28 under GAL1 promoter in yeast

Depositor

Charles Brenner

Article

Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

Insert

TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)

Expression

Yeast

Promoter

Gal1

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only
pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

Plasmid

#188704

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 S392A

Plasmid

#25976

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
pBS cErm (CT#1054)

Plasmid

#13907

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Erm (ETV5 Chicken)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
p3039 pVL E6-AP

Plasmid

#8646

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Insect

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
Cas-Acr (D14A/G38A)

Plasmid

#128118

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (D14A/G38A)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBS cCollagen XII (CT#1051)

Plasmid

#13916

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Collagen XII (COL12A1 Chicken)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

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p3004 pGEM E6-AP 76kD

Plasmid

#10857

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Mutation

76kD: amino acids 213-865

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pBS cFgfr2 (CT#221)

Plasmid

#14003

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Fgfr2 (FGFR2 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
p3012 pGEM E6-AP C-A

Plasmid

#8645

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Bacterial

Mutation

active site cyteine codon mutated to encode alani…

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pBS cPea3 (CT#1024)

Plasmid

#13906

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (ETV4 Chicken)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
p3045 pVL E6-AP C-A

Plasmid

#8647

Depositor

Peter Howley

Article

Huibregtse et al Mol Cell Biol 1993 Aug;13(8):4918-27.

Insert

E6-AP (UBE3A Human)

Expression

Insect

Mutation

Active site Cys mutated to encode Ala

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pJV457

Plasmid

#180080

Purpose

M.BbrUI expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUI

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJV461

Plasmid

#180081

Purpose

M.BbrUIII expression construct to improve transformation in B. breve UCC2003

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Insert

M.BbrUIII

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE

Plasmid

#233264

Purpose

Backbone plasmid used to clone lncRNA inserts in preparation for RMCE

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Type

Empty backbone

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3GFP

Plasmid

#233008

Purpose

Galactose iduced expression of Gcn4 E+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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