We narrowed to 5,035 results for: mcs

1. pAi14-GFPNLS-MC

   Plasmid

   #87114

   Purpose

   minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   GFPNLSPpA
   

   Use

   CRISPR

   Promoter

   nEF

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDOE20 mC VN

   Plasmid

   #124246

   Purpose

   Allows fluorescent (mVENUS; mCherry) co-localization of two proteins in Agrobacterium- transformed plant cells.

   Depositor

   Attila Fehér

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdTrack-mC/EBPbeta

   Plasmid

   #49196

   Purpose

   adenoviral shuttle vector with mouse C/EBPbeta

   Depositor

   Jed Friedman

   Insert

   C/EBPbeta LAP fragment (Cebpb Mouse)
   

   Use

   Adenoviral

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMTB-Multibow-mC

   Plasmid

   #60986

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound cerulean

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   Cerulean
   

   Use

   Zebrafish

   Tags

   membrane tag

   Promoter

   Bactin2

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. scAV0.1-TBG-mC

   Plasmid

   #192879

   Purpose

   expression of mCherry

   Depositor

   Kazuhiro Oka

   Insert

   mCherry
   

   Use

   AAV

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

   Plasmid

   #69909

   Purpose

   Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

   Plasmid

   #149276

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

   Plasmid

   #199349

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200402

   Purpose

   Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Closed-loop DART VADAR sensor entry cassette
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

    Plasmid

    #197989

    Purpose

    For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

    Depositor

    Bo Wang

    Article

    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

    Insert

    HSALNT0217290
    

    Use

    Lentiviral

    Tags

    lac

    Expression

    Mammalian

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

    Plasmid

    #84467

    Purpose

    Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Type

    Empty backbone

    Use

    AAV

    Promoter

    pRAM and TRE

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

    Plasmid

    #199359

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

    Plasmid

    #199350

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Promoter

    Cbr-unc-119p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

    Plasmid

    #199348

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103]

    Plasmid

    #16283

    Depositor

    Thomas Jessell

    Article

    Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

    Insert

    HB9 promoter (Mnx1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

18. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

    Plasmid

    #149277

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid

    #176277

    Purpose

    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-mCherry
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin I

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES

    Plasmid

    #69910

    Purpose

    Expresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge