We narrowed to 10,063 results for: Uty

1. pInducer20_SLC38A2_wt

   Plasmid

   #156182

   Purpose

   Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   TRE

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-PHB-4

   Plasmid

   #122231

   Purpose

   Expresses shRNA targeting the 3' UTR of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   PHB (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET.SUMO eIF4G1 +MIC 1-200

   Plasmid

   #158792

   Purpose

   Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (with microexon) codon optimized
   

   Tags

   6xHIS and SUMO

   Expression

   Bacterial

   Mutation

   amino acids 1-200

   Promoter

   T7

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. px335 Mettl14 sgRNA #1

   Plasmid

   #61515

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gcggcagctcctagctcagc
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMEXneo-TrkC-TrkB-hybrid

   Plasmid

   #190205

   Purpose

   To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Expression

   Mammalian

   Mutation

   The second IgG like domain and the extracellular …

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiRFP-H2B-C1

   Plasmid

   #108411

   Purpose

   Histone 2B fused to monomeric near-infrared fluorescent protein miRFP

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   miRFP-H2B (H2BC21 Human)
   

   Tags

   miRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEXSISERS_FLuc_loxP-PuroR

   Plasmid

   #177866

   Purpose

   Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

   Insert

   FLuc-based EXSISERS
   

   Use

   CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3E-hGPR56 e1m promoter normal

   Plasmid

   #52298

   Purpose

   Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
   

   Use

   Luciferase

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR207 SARS-CoV-2 NSP1 124A/125A

   Plasmid

   #164523

   Purpose

   Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Insert

   NSP1 124A/125A (ORF1ab )
   

   Expression

   Mammalian

   Mutation

   Insert of NSP1 gene's CDS from SARS-CoV-2 is…

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-TRC.mKO2_shGLS

    Plasmid

    #110319

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GLS Glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-PHB-6

    Plasmid

    #122232

    Purpose

    Expresses shRNA targeting the coding sequence of human PHB

    Depositor

    Alex Kentsis

    Article

    Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

    Insert

    shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS1401

    Plasmid

    #29198

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGG369

    Plasmid

    #165606

    Purpose

    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

    Expression

    Bacterial

    Mutation

    Recoding of BsaI site in the AmpR cassette, two B…

    Promoter

    lacUV2 driving ω-dCas9 and UV5 driving sgRNA

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCMVblast_SLC38A2_N82A

    Plasmid

    #156181

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    CMV

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pInducer20_SLC38A2_N82A

    Plasmid

    #156183

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

    Plasmid

    #156405

    Purpose

    Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mScarlet-I

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 rtTA BirA eIF4G

    Plasmid

    #158789

    Purpose

    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag and BirA*

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid

    #52299

    Purpose

    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Mutation

    15-bp deletion in the promoter

    Promoter

    Human GPR56 e1m promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1538

    Plasmid

    #29261

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Oct. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTW334e2b

    Plasmid

    #246587

    Purpose

    sequential knock out of A. thaliana Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)
    

    Expression

    Bacterial

    Mutation

    AtBSD2W108A/L109E

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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