We narrowed to 32,650 results for: Eng

1. pLV Cbx5-V5

   Plasmid

   #175105

   Purpose

   Lentiviral expression of mouse Cbx5-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Cbx5 (Cbx5 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ai27

   Plasmid

   #34630

   Purpose

   Cre-dependent expression of hChR2(H134R)-tdTomato, for optogenetic excitation

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

   Insert

   Floxed hChR2(H134R)-tdTomato
   

   Use

   Mouse Targeting

   Tags

   tdTomato

   Mutation

   His 134 has been mutated to Arg

   Promoter

   CAG

   Available Since

   Feb. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC95-pmax-dCas9VP160-2A-neo

   Plasmid

   #48227

   Purpose

   dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
   

   Use

   CRISPR

   Tags

   2A-neo, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. plko-shmfn2-mfn2-Rescue

   Plasmid

   #160655

   Purpose

   "Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   mfn2 (MFN2 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBZCas13b-HEPN

   Plasmid

   #89901

   Purpose

   Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Expression

   Bacterial

   Mutation

   R116A/H121A/R1177A/H1182A

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHRIG-PRAS40

   Plasmid

   #53599

   Purpose

   lentiviral expression of constitutively active PRAS40 and EGFP

   Depositor

   Heng Zhao

   Article

   Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

   Insert

   PRAS40 (AKT1S1 Human)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP and myc

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. AAV-CaMKII-TurboID

   Plasmid

   #227178

   Purpose

   Co-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vector

   Depositor

   Junmin Peng

   Article

   Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

   Insert

   TurboID
   

   Use

   AAV

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CaMKIIa_short

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV Gpat4-V5

   Plasmid

   #175145

   Purpose

   Lentiviral expression of mouse Gpat4-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Gpat4 (Gpat4 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. human ETV6 gRNA-1

   Plasmid

   #133353

   Purpose

   human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

   Depositor

   Linzhao Cheng

   Article

   Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

   Insert

   ETV6 (ETV6 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMYs-MFN2-IRES-mitoRFP

    Plasmid

    #121997

    Purpose

    Expression of MFN2 and mitoRFP in mammalial cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    MFN2-IRES-mitoRFP (MFN2 Human)
    

    Use

    Retroviral

    Mutation

    silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-MBP-(CBX1)3-WT

    Plasmid

    #180212

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1 x3 (CBX1 Human)
    

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. BE3-hA3A-R128A

    Plasmid

    #132944

    Purpose

    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-hA3A(R128A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-hA3A(R128A)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 and CMV

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80066

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Beta-actin2

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-NLC-DHFR-SpCas9

    Plasmid

    #124523

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NLC-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. tdEos-Vimentin-7

    Plasmid

    #57688

    Purpose

    Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Vimentin (VIM Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80060

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Myl7 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170996

    Purpose

    HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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