We narrowed to 14,016 results for: pcdna

1. pcDNA3.1V5-His Snk/Plk2 D223N

   Plasmid

   #22218

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, aspartic a…

   Promoter

   Available Since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

   Plasmid

   #69899

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (stw Fly, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-GFP-GFP-FLARE-AKAR

   Plasmid

   #123332

   Purpose

   Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   GFP-GFP-FLARE-AKAR
   

   Use

   Tags

   6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA1 Flag ATXN1[85Q]-S776A

   Plasmid

   #33240

   Depositor

   Huda Zoghbi

   Article

   Lam et al Cell. 2006 Dec 29;127(7):1335-47.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 85 Glutamines and no…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)

   Plasmid

   #29438

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 267-1556 (a.a.) E653Q

   Promoter

   Available Since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nano-lantern(Ca2+)_117/pcDNA3

   Plasmid

   #51985

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

   Plasmid

   #168188

   Purpose

   HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-nUbc6E-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NC4 pCDNA3.1 VENUS WTX (1-804)

   Plasmid

   #36959

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   WTX (AMER1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-Venus-cpVenus-FLARE-CKAR

   Plasmid

   #123343

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-KozATG-dH6.2-2XG4S-actin

    Plasmid

    #73263

    Purpose

    Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)

    Depositor

    Marcel Bruchez

    Article

    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

    Insert

    KozATG-dH6.2-2XG4S-actin (ACTB Human, Synthetic)
    

    Use

    Tags

    The FAP and actin are fused with 2 copies of a G4…

    Expression

    Mammalian

    Mutation

    The dH6.2 FAP has one cysteine in each domain cha…

    Promoter

    CMV

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

    Plasmid

    #171431

    Purpose

    Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    hH2A-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Flag-RNF168 delta MIU2

    Plasmid

    #133979

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU2

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

    Plasmid

    #46670

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-let-7a-1 (MIRLET7A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cer(1-158)-gamma-5 in pcDNAI/Amp

    Plasmid

    #55625

    Purpose

    An amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    Cer(1-158)-gamma-5 (GNG5 Aequorea victoria, Human)
    

    Use

    Tags

    Cer(1-158) was fused to the amino terminus of Gga…

    Expression

    Mammalian

    Mutation

    Ggamma-5 was amplified by PCR, which added a BamH…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid

    #54470

    Purpose

    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Promoter

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc3-CUL4A delta N100

    Plasmid

    #19953

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CUL4A (cullin 4A) (CUL4A Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    The longest published clone (Q13619)

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA4/TO/FLAG-hPMR1-Tev-Bio

    Plasmid

    #80125

    Purpose

    Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

    Depositor

    Daniel Schoenberg

    Article

    Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

    Insert

    PXDNL-003 (PXDNL Human)
    

    Use

    Tags

    FLAG and biotinylation

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-BirA-FLAG-N-term-HDLBP

    Plasmid

    #154453

    Purpose

    Expression of N-term BirA tagged HDLBP

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    HDLBP (HDLBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA8-Flag-LARS-DELTA-UNE-L

    Plasmid

    #139691

    Purpose

    Expresses N-teminal Flag tagged aa 1- 1064 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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