We narrowed to 80,379 results for: TRI

1. pSH-EFIRES-P-GFP(1-10)opti

   Plasmid

   #129416

   Purpose

   Expressing codon-optimized GFP(1-10) fragment in human cells

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   codon-optimized GFP(1-10)
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1_mGFP-FGFR3-K508M

   Plasmid

   #191753

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1523A>T (p.K508M) + silent mutation c.1515A&…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

   Plasmid

   #149714

   Purpose

   Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   BHLHE40 (BHLHE40 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNK5867

   Plasmid

   #219751

   Purpose

   pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - mcitHispS - tAOX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_mGFP-FGFR3-K650Q

   Plasmid

   #191756

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>C (p.K650Q) + silent mutation c.1938C&…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC-BAR-YR

   Plasmid

   #61766

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Bar gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOPINB-hRNF216(511-784)

   Plasmid

   #171923

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

   Plasmid

   #161577

   Purpose

   Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid

    #184562

    Purpose

    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    NPRL2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines.

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JWW-2 human chimeric monoclonal antibody

    Plasmid

    #66749

    Purpose

    Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-2 Heavy Chain
    JWW-2 Light Chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 and rEF1

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe RFP1-KRT5 hygro

    Plasmid

    #58493

    Purpose

    mammalian expression of mRFP1-tagged human KRT5

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    KRT5 (KRT5 Human)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pIRES2-DsRed-MST-Kv2.1

    Plasmid

    #131707

    Purpose

    Expresses Kv2.1in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

    Insert

    KCNB1 (KCNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIRES2-EGFP-p53 tethered

    Plasmid

    #49245

    Purpose

    expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53-p53 (TP53 Human)
    

    Use

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

    Plasmid

    #126697

    Purpose

    donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126698

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

    Insert

    sgRNA targeting human SCGB3A2 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-SwitchON-sgVRK1

    Plasmid

    #199638

    Purpose

    Tamoxifen-inducible expression of sgRNA targeting VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEMS-Tom20-mEGFP-reHaloTagS

    Plasmid

    #200189

    Purpose

    Tom20 with reversible HaloTagS variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mEGFP and reHaloTagS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge