167,318 results

1. Nb-gp41-Halo (MoonTag-Nb-Halo)

   Plasmid

   #128603

   Purpose

   Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and Halo

   Promoter

   SFFV

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTNS1

   Plasmid

   #64967

   Purpose

   Tn7 transposase expression

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   tnsABCD
   

   Available Since

   Oct. 23, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRK-ATF4

   Plasmid

   #26114

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5HRE/GFP

   Plasmid

   #46926

   Purpose

   hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

   Depositor

   Thomas Foster, Martin Brown

   Article

   Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

   Insert

   5X HRE of VEGF (VEGFA Human)
   

   Tags

   d2EGFP

   Expression

   Mammalian

   Mutation

   five copies of a 35-bp fragment from the hypoxia-…

   Promoter

   EF-1α promoter

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTK_Citrine_Nanotag_24_positive_Control

   Plasmid

   #130570

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   FoxD3_cranial_neural_crest_enhancer_NC1
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_NOTCH2_WT

   Plasmid

   #82292

   Purpose

   Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NOTCH2 (NOTCH2 Human)
   

   Use

   Gateway entry vector

   Mutation

   C946Y

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVAX1/mTyr-Cre

   Plasmid

   #99249

   Purpose

   Expresses Cre recombinase under tyrosinase promoter

   Depositor

   Christian Blank

   Article

   Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

   Inserts

   Cre recombinase
   mTyr enh/prom
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLOX-TERT-iresTK

   Plasmid

   #12245

   Purpose

   2nd generation lentiviral vector for reversible immortalization of human primary cells

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   TERT, HSV1-TK (TERT Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-6His-DHX9

   Plasmid

   #211440

   Purpose

   generation of Bacmid for insect cell expression of DHX9 N-terminally fused to 6XHis-Tag

   Depositor

   Helmut Pospiech, Frank Grosse

   Article

   Zhang et al J Biol Chem. 1997 Apr 25. 272(17):11487-

   Insert

   DHX9 (DHX9 Human)
   

   Tags

   6xHis

   Expression

   Insect

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP1

    Plasmid

    #79873

    Purpose

    Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

    Depositor

    Stanley Qi, Jason Peters, Carol Gross

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    xylA

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 7TFC

    Plasmid

    #24307

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-FFluc // SV40-mCherry
    

    Use

    Lentiviral

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCerulean3-N1

    Plasmid

    #54730

    Purpose

    Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Tags

    mCerulean3

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available Since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP rbFOX2

    Plasmid

    #63086

    Purpose

    Mammalian expression of EGFP tagged FOX2

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCrhaB2

    Plasmid

    #113634

    Purpose

    An expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepacia

    Depositor

    Miguel Valvano

    Article

    Cardona et al Plasmid. 2005 Nov;54(3):219-28. doi: 10.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pECE

    Plasmid

    #26453

    Depositor

    William Rutter

    Article

    Ellis et al Cell. 1986 Jun 6;45(5):721-32.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. Golgi-TurboID

    Plasmid

    #200959

    Purpose

    Transiently expresses golgi localized TurboID with HA tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBA615

    Plasmid

    #186239

    Purpose

    eLbuCas13a Negative Control (RFP targeting)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    pTet-LbuCas13a + crRNA negative control
    

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. 765 pME nls mCerulean

    Plasmid

    #195958

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAS165

    Plasmid

    #194488

    Purpose

    97-4 Op (Part 2)

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    97-4 Op (Part 2)
    

    Use

    Synthetic Biology

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-R26-FLEX-mStr

    Plasmid

    #135619

    Purpose

    Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. phOct4-EGFP

    Plasmid

    #38776

    Depositor

    Wei Cui

    Article

    Gerrard et al Stem Cells. 2005;23(1):124-33.

    Insert

    Oct-4 promoter (POU5F1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    human OCT4

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #86613

    Purpose

    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLenti6MQBI163 SGLT2

    Plasmid

    #194147

    Purpose

    Expresses SGLT2 protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC5A2 (SLC5A2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRRlsinPGK_H2BGFP_WPRE

    Plasmid

    #91788

    Purpose

    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    Human histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge