We narrowed to 12,145 results for: SHA

Showing: 6101 - 6120 of 12145 results

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pSpCas9(BB)-2A-GFP-G3BP2(1)

Plasmid

#136060

Purpose

G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP2 (G3BP2 Human)

Expression

Mammalian

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Tags

EYFP, His-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip2

Plasmid

#59027

Purpose

act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

Depositor

Roger Davis

Article

Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Insert

mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

Plasmid

#197952

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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mEmerald-H2B-C-10

Plasmid

#54112

Purpose

Localization: Nucleus/Histones, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

H2B (H2BC11 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMM9-MAPKK2-WT

Plasmid

#40805

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Tags

HA

Expression

Mammalian

Mutation

Wild type

Promoter

MSV-LTR

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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px552-sg-gria1-HT-SEP

Plasmid

#187653

Purpose

Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

HaloTag-SEP donor

Use

AAV and CRISPR

Tags

N/A

Promoter

N/A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GST

Plasmid

#41611

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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Clover-PDHA1-N-10

Plasmid

#56304

Purpose

Localization: Mitochondria, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PDHA1 (PDHA1 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Flag-ECFP-GIT1

Plasmid

#15223

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

ECFP and flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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GalT-moxDendra2

Plasmid

#89789

Purpose

mammalian expression of GC localized moxDendra2

Depositor

Erik Snapp

Article

Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

Insert

GalT signal anchor transmembrane domain (B4GALT1 Human)

Tags

moxDendra2

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBLO2 casX

Plasmid

#123122

Purpose

E. coli expression vector for CasX sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX guide RNA

Use

CRISPR

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-hTRAF3 1-448

Plasmid

#44033

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

Contains amino acids 1-448; V274D and S393G

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_Z3_Gal4VP64

Plasmid

#169916

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch core and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS433-pCSDest2-AcrIIC1Nme

Plasmid

#85679

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC1Nme

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mRuby2-Nup50-N-10

Plasmid

#55908

Purpose

Localization: Nuclear Pore, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Nup50 (NUP50 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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