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pENTR L1-5XQUAS-L4

Plasmid

#32308

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

5XQUAS

Use

Gateway multisite entry clone

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR L1-5XQUAS-R5

Plasmid

#32303

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

5XQUAS

Use

Gateway multisite entry vector

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR L1-20XUAS-L4

Plasmid

#32307

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

20XUAS

Use

Gateway multisite entry clone

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR L5-GAL4-L2

Plasmid

#32304

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

GAL4 (GAL4 Budding Yeast)

Use

Gateway multisite entry clone

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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MSGV Mouse Acceptor PGK NGFR

Plasmid

#128482

Purpose

A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Promoter

MSCV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB2b

Plasmid

#19524

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2b (DNAJB2 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB5

Plasmid

#19500

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB5 (DNAJB5 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL30009

Plasmid

#50301

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal Myc tag (4x Myc)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR P4-P1R-EGFP

Plasmid

#48348

Purpose

Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway Cloning

Mutation

Contains a Kozak sequence. Does not contain a sto…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EGFP

Plasmid

#48349

Purpose

Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway Cloning

Mutation

Aminoacid 40 is valine in our sequence, while it …

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-ECFP

Plasmid

#48353

Purpose

Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

ECFP

Use

Gateway Cloning

Mutation

Contains a stop codon.

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSHDY

Plasmid

#137661

Purpose

Conjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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IZ501: pMVP/Lenti-DEST

Plasmid

#121848

Purpose

pMVP destination vector, empty Lentivirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Kan)

Plasmid

#110081

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Hyg)

Plasmid

#110082

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMU102

Plasmid

#61805

Purpose

Cloning and expression vector for Clostridium thermocellum

Depositor

Lee Lynd

Article

Olson et al J Biol Eng. 2012 May 11;6(1):5. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-iRFP670-Tdtomato-GFP

Plasmid

#111817

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
Tdtomato
GFP

Use

Cloning intermediate

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-PT2A-iRFP670-GFP-Tdtomato

Plasmid

#111816

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
GFP
Tdtomato

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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