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pLenti-EGFP-2xFYVE

Plasmid

#136996

Purpose

Lentiviral EGFP lipid sensor for PI(3)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-2xFYVE

Use

Lentiviral

Tags

EGFP

Promoter

EF1 alpha

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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T7-CMVtrans-FFLuc-polyA

Plasmid

#101156

Purpose

Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

Depositor

Marcel Bruchez

Article

Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

Insert

Firefly luciferase

Use

Luciferase

Promoter

T7 promoter

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV puro let-7 sponge

Plasmid

#29766

Depositor

Phil Sharp

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

let-7 sponge

Use

Retroviral

Expression

Mammalian

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
C32V

Plasmid

#26396

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-32-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-TEAD2

Plasmid

#33107

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD2 (TEAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-oChIEF-tdTomato

Plasmid

#50977

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-tdTomato

Use

AAV

Tags

tdTomato

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/VChR1-EYFP

Plasmid

#20955

Purpose

Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

Depositor

Karl Deisseroth

Article

Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

Insert

volvox channelrhodopsin-1

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pDZ415 (24MS2SL loxP-Kan-loxP)

Plasmid

#45162

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xMS2 Stem Loop Cassette

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCLucf

Plasmid

#37328

Depositor

John Schiller

Article

Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

Inserts

Luciferase (luciferase Fly)
GFP

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Article

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec24C

Plasmid

#66608

Purpose

YFP fusion of Sec24C

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24C (SEC24C Human)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-MKL2

Plasmid

#27175

Purpose

For mammalian expression of human MKL2 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Selvaraj et al J Biol Chem. 2003 Oct 24. 278(43):41977-

Insert

MKL2 (MRTFB Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCMV Sport2 mPer2

Plasmid

#16204

Depositor

Cheng Lee

Article

Albrecht et al Cell. 1997 Dec 26. 91(7):1055-64.

Insert

Per2 (Per2 Mouse)

Expression

Mammalian

Available Since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only
CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pWPI_SPbFGF

Plasmid

#25812

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

pWPI_SPbFGF (FGF2 Human)

Use

Lentiviral

Mutation

Ig signal peptide in N-terminal of human FGF2

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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