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Showing: 1201 - 1240 of 4626 results

  1. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid
    #198539
    Purpose
    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-APOBEC-nCas9-Ung
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198539-plasmid-map-sequence-id-388890

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  3. Sniper2P

    Plasmid
    #193857
    Purpose
    Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193857-plasmid-map-sequence-id-382456

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  7. pPKm-240

    Plasmid
    #90510
    Purpose
    pSIN - EF-1alpha - Cyto -sFd - P2A - Cyto - sFNR, vector encoding for cytoplasmic-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoter
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    cyto-sFD, cyto-sFNR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    90510-plasmid-map-sequence-id-204691

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  8. pEGFP-N1-Letm1

    Plasmid
    #191937
    Purpose
    Expression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera.
    Depositor
    Luca Scorrano
    Insert
    Letm1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191937-plasmid-map-sequence-id-379512

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  9. EFR WT

    Plasmid
    #102413
    Purpose
    fluorescent tagging. Plant expression of EFR WT
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102413-plasmid-map-sequence-id-197797

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  10. pEGFP-N1-Letm1

    Plasmid
    #191937
    Purpose
    Expression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera.
    Depositor
    Luca Scorrano
    Insert
    Letm1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191937-plasmid-map-sequence-id-379512

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  11. EFR WT

    Plasmid
    #102413
    Purpose
    fluorescent tagging. Plant expression of EFR WT
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102413-plasmid-map-sequence-id-197797

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  12. pCDH-EF1-DIO-copGFP

    Plasmid
    #72253
    Purpose
    Express copGFP under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72253-plasmid-map-sequence-id-131724

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  13. lentiGuide_puro-P2A-mNeonGreen

    Plasmid
    #221464
    Purpose
    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
    Depositor
    James Eshleman
    Insert
    mNeonGreen
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6, EF-1a
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    221464-plasmid-map-sequence-id-438191

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  14. pCDH-EF1-DIO-copGFP

    Plasmid
    #72253
    Purpose
    Express copGFP under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72253-plasmid-map-sequence-id-131724

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  15. lentiGuide_puro-P2A-mNeonGreen

    Plasmid
    #221464
    Purpose
    Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker
    Depositor
    James Eshleman
    Insert
    mNeonGreen
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6, EF-1a
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    221464-plasmid-map-sequence-id-438191

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  16. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  17. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  18. EFR Y877F (17)

    Plasmid
    #102415
    Purpose
    fluorescent tagging. Plant expression of EFR Y877F (17)
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y877F
    Promoter
    35S
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    102415-plasmid-map-sequence-id-197842

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  19. EFR Y875F (15)

    Plasmid
    #102414
    Purpose
    fluorescent tagging. Plant expression of EFR Y875F (15)
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y875F
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102414-plasmid-map-sequence-id-197801

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  20. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  21. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  22. EFR Y877F (17)

    Plasmid
    #102415
    Purpose
    fluorescent tagging. Plant expression of EFR Y877F (17)
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y877F
    Promoter
    35S
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    102415-plasmid-map-sequence-id-197842

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  23. EFR Y875F (15)

    Plasmid
    #102414
    Purpose
    fluorescent tagging. Plant expression of EFR Y875F (15)
    Depositor
    Silke Robatzek
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y875F
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102414-plasmid-map-sequence-id-197801

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  24. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Martin Carroll
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Expression
    Mutation
    Promoter
    EFS (P2A)
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187646-plasmid-map-sequence-id-367454

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  25. pXPR_016

    Plasmid
    #107143
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107143-plasmid-map-sequence-id-205293

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  27. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  38. lentiGuide-Hygro-eGFP

    Plasmid
    #99375
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99375-plasmid-map-sequence-id-191025

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  39. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99374-plasmid-map-sequence-id-430620

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  40. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179543-plasmid-map-sequence-id-354769

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Showing: 1201 - 1240 of 4626 results