Addgene

We narrowed to 813 results for: PC1

Showing: 601 - 620 of 813 results

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pGEX6-Smad3 EPSM V179T

Plasmid

#27015

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; V179T

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A208S

Plasmid

#27109

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A208S

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM A213S

Plasmid

#27110

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A; A213S

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC AAGY

Plasmid

#27039

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; P181A P182A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

P181A P182A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
MAC_N_COMMD9

Plasmid

#209681

Purpose

Expresses MAC-tagged COMMD9 in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

COMMD9 (COMMD9 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-MYO10ΔF3

Plasmid

#194857

Purpose

Express EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF3 (MYO10 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncated MYO10 construct. MYO10 amino acids 1-1…

Promoter

CMV

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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SAE1

Plasmid

#175241

Purpose

Bacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimer

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SAE1 (SAE1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCR1319

Plasmid

#111116

Purpose

Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

UBE2T gRNA (UBE2T Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1320

Plasmid

#111117

Purpose

Expresses UBE2T_-_202311067.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

UBE2T gRNA (UBE2T Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Flag Smad3 AAGY

Plasmid

#27027

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-TRPM1 cDNA

Plasmid

#29782

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

TRPM1 (TRPM1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta WT GFP

Plasmid

#128820

Purpose

To visualize Doc2beta WT in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-RNF68

Plasmid

#126545

Purpose

Expresses mCherry-tagged RNF68 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68

Plasmid

#126548

Purpose

Expresses FLAG-HA-tagged RNF68 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pKG139

Plasmid

#69755

Purpose

Retroviral expression of CENP-C

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-C (CENPC Human)

Use

Retroviral

Tags

mCherry-TEV-S

Expression

Mammalian

Mutation

L341F, N570D relative to NP_001803.2 (numbering b…

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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S1PR4-Tango

Plasmid

#66499

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

S1PR4 (S1PR4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD9 Flag

Plasmid

#74901

Purpose

Express COMMD9 in mammalian cells

Depositor

Colin Duckett, Ezra Burstein

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD9 (COMMD9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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