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pdCas9-GFP-VPR

Plasmid

#181907

Purpose

Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9-VPR

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1alpha

Plasmid

#181900

Purpose

Fluorescently tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) eGFP

Plasmid

#107505

Purpose

Expresses eGFP, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1gamma

Plasmid

#181902

Purpose

Fluorescently tagged HP1gamma

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1gamma (Cbx3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC14A1_STOP

Plasmid

#161123

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC14A1 (SLC14A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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alpha actinin delta ABD-GFP

Plasmid

#66935

Purpose

Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

Depositor

Pere Roca-Cusachs, Michael Sheetz

Article

Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Alpha-actinin 1 (ACTN1 Human)

Tags

GFP

Expression

Mammalian

Mutation

actin binding domain (amino acids 30–253) is dele…

Promoter

CMV

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pMECP2-GFP

Plasmid

#181904

Purpose

Fluorescently tagged MECP2, GFP

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

MECP2 (Mecp2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-ELK1

Plasmid

#98604

Purpose

Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 (Elk1 Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Bacterial

Promoter

T7

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FITM2

Plasmid

#107504

Purpose

Expresses FITM2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FITM2 (codon optimized)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 SIRT1-deltaExon2

Plasmid

#105671

Purpose

Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUC_hU6-shRKIP_BFP

Plasmid

#199216

Purpose

Expression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

shBACH1 (BACH1 Synthetic)

Use

RNAi

Promoter

human U6

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-mpx

Plasmid

#191826

Purpose

mpx probe generation for in situ

Depositor

Jason Berman

Article

Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

Insert

mpx cDNA (mpx Zebrafish)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMECP2-RFP

Plasmid

#181905

Purpose

Fluorescently tagged MECP2, RFP

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

MECP2 (Mecp2 Mouse)

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE3G-ZSGreen1-mHP1alpha

Plasmid

#181913

Purpose

Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

ZSGreen1

Expression

Mammalian

Promoter

TRE3G

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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Phage-PGK-Flag-HA-FKBP-T(G177D)

Plasmid

#181735

Purpose

for lentiviral expression of brachyury ORF in mammalian cells

Depositor

Charles Lin

Article

Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

Insert

T (brachyury)

Use

Lentiviral

Mutation

a silent mutation in the PAM site of an sgRNAt ta…

Available Since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

Plasmid

#105681

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-NKX2-5Y191C

Plasmid

#98598

Purpose

Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 (Nkx2-5 Mouse)

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Mutation

Y191C point mutant

Promoter

Heat Shock Minimal Promoter

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLgw NKX2-5Y191C-V5-EcoDam

Plasmid

#98599

Purpose

Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 (Nkx2-5 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Mutation

Y191C point mutant

Promoter

Heat Shock Minimal Promoter

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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