We narrowed to 8,552 results for: pcDNA3

1. POLG-pcDNA3.1

   Plasmid

   #191487

   Purpose

   Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.

   Depositor

   Hans Zempel

   Insert

   POLG (POLG Human)
   

   Use

   Tags

   V5 tag, 6X His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-J chain

   Plasmid

   #145146

   Purpose

   Mammalian expression plasmid for J chain of polymeric antibody classes

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   immunoglobulin J chain (JCHAIN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hRXRα

   Plasmid

   #135400

   Purpose

   Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using CMV promoter

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoid X receptor- alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3 WT B-Myb

   Plasmid

   #25965

   Depositor

   Rob Lewis

   Article

   Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

   Insert

   B-Myb (MYBL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. GZnP2 in pcDNA3.1+

   Plasmid

   #161739

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

   Insert

   GZnP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-SOX4

   Plasmid

   #110367

   Purpose

   Mammalian expression of HA-tagged SOX4

   Depositor

   Carlos Moreno

   Insert

   SOX4 (SOX4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-GAVPO

   Plasmid

   #159923

   Purpose

   GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

   Depositor

   Fei Chen

   Article

   Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

   Insert

   GAVPO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hfYFP

   Plasmid

   #186523

   Purpose

   Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-hFrataxin-HA

    Plasmid

    #31895

    Depositor

    Gino Cortopassi

    Article

    Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

    Insert

    Frataxin (FXN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-MEK5DD-HA

    Plasmid

    #65247

    Purpose

    Expression of HA tagged dominant active MEK5 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

    Insert

    MEK5DD (MAP2K5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Ser311 to Asp, Ser315 to Asp

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA-eEF2K

    Plasmid

    #110160

    Purpose

    Expression of human eEF2K in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

    Insert

    eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-mPCSK4

    Plasmid

    #122675

    Purpose

    Expresses mouse PCSK4 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ER-GCaMP3

    Plasmid

    #64854

    Purpose

    Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-GCaMP3
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mADAM17 in pcDNA3.1

    Plasmid

    #19141

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)- e18

    Plasmid

    #131670

    Purpose

    Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-N-I-N-CAAX

    Plasmid

    #41686

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TYMS

    Plasmid

    #100545

    Purpose

    mammalian expression of thymidylate synthetase

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

    Insert

    thymidylate synthetase (TYMS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Shank2-HA /pCDNA3

    Plasmid

    #197334

    Purpose

    Plasmid expressing an antigen targeting Shank2

    Depositor

    Morgan Sheng

    Insert

    Shank2 (Shank2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ps-CFP

    Plasmid

    #13034

    Purpose

    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence

    Depositor

    Doug Golenbock

    Insert

    photo-switchable cyan fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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