We narrowed to 8,188 results for: pcas

1. pCAT-YFP-TetR

   Plasmid

   #59018

   Purpose

   Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

   Depositor

   David Sibley

   Article

   Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

   Insert

   YFP-TetR
   

   Use

   Toxoplasma expression

   Promoter

   alpha-tubulin

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT224_(pCA-tTA2)

   Plasmid

   #36430

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tetracycline transactivator
   

   Expression

   Mammalian

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-6His-mISG15

   Plasmid

   #12445

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Tags

   6xHis

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-HkCas12a-2AeGFP

   Plasmid

   #123634

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   HkCas12a
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Flp-DOG

   Plasmid

   #80084

   Purpose

   Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   Flp-DOG
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Gal4DBD-GBP2

   Plasmid

   #49439

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP2-Gal4 DNA binding domain fusion protein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG tubulin GFP

   Plasmid

   #66105

   Purpose

   expression of chicken tubulin GFP

   Depositor

   Martin Scaal, Felicitas Proels

   Article

   et al Development. 2015 Feb 15;142(4):665-71.

   Insert

   tubulin
   

   Use

   Chicken expression

   Tags

   EGFP

   Promoter

   CAG

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG vGlut1 GCaMP5G

    Plasmid

    #105008

    Purpose

    Presynaptic calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-CD4-myc

    Plasmid

    #58537

    Purpose

    Expresses human CD4 with a C-terminal myc tag in mammalian cells

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBW842_pCAG-pExpr-FP-DECODER

    Plasmid

    #87551

    Purpose

    Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP, EGFP, iRFP720, mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-p65AD-GBP6

    Plasmid

    #49440

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP6-p65 transactivation domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCasper_Pdf-LexA::VP16

    Plasmid

    #33259

    Depositor

    Michael Rosbash

    Article

    Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

    Insert

    LexA::VP16-SV40 3'UTR (lexA E. coli)
    

    Expression

    Insect

    Promoter

    Pdf

    Available Since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCAGGS-PSD95-GCaMP2

    Plasmid

    #18931

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    PSD95-GCaMP2 (Dlg4 Rat)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCAX APP C60

    Plasmid

    #30152

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein C60
    

    Expression

    Mammalian

    Mutation

    fragment

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-sREACh-Actin

    Plasmid

    #21949

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

    Insert

    betaActin
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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