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AiP14478 - pAAV-AiE0456m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4478)

Plasmid

#229923

Purpose

AiE0456m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-RECQL5

Plasmid

#222620

Purpose

Mammalian expression vector of flag-tagged RECQL5

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

RECQL5 (RECQL5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

Plasmid

#216533

Purpose

An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

Depositor

Yuki Kambe

Article

Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

Insert

axon-jGCaMP8m

Use

AAV and Cre/Lox

Tags

GAP43

Expression

Mammalian

Promoter

hSynapsin1

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS

Plasmid

#199353

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-TEV

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-xMBP-ACE2-CHis

Plasmid

#194998

Purpose

Production of ACE2 extracellular domain

Depositor

Marko Hyvönen

Article

Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

Insert

ACE2 (ACE2 Human)

Tags

His8 and xMBP

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PRPF4

Plasmid

#155850

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF4 (PRPF4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PRPF3

Plasmid

#155848

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF3 (PRPF3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NAP1L1-Halo

Plasmid

#175342

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NAP1L1 (NAP1L1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDOC-K-glmS

Plasmid

#158058

Purpose

Donor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable marker

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Inserts

glmS homologous region 1
glmS homologous region 2 + MCS3

Use

Synthetic Biology

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pASPIre2

Plasmid

#154843

Purpose

Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1

Depositor

Markus Jeschek

Article

Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

Insert

Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon

Expression

Bacterial

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-CDC42-DN

Plasmid

#109584

Purpose

Multisite gateway vector for 3' tagging with dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-lamp1-mKate2

Plasmid

#109507

Purpose

Muscle specific zebrafish expression of mKate2 fused to lamp1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

lamp1 (lamp1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP2

Plasmid

#106409

Purpose

P-RAN-RFP2 (RANbody RFP2::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP2

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-Gelsolin

Plasmid

#106413

Purpose

P-RAN-Gelsolin (RANbody Gelsolin::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-Gelsolin

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-GFP

Plasmid

#105946

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC (Prkcz Rat)

Tags

CAAX and GFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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