We narrowed to 17,340 results for: EMB

1. His6_BcLOV4_C292A_mCherry_BamUK

   Plasmid

   #119761

   Purpose

   Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

   Depositor

   Brian Chow

   Article

   Glantz et al Methods Enzymol. 2019;622:249-270. doi:

   Insert

   BcLOV4-C292A_mCherry
   

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Mutated cysteine at position 292 to alanine

   Promoter

   T7

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C453-E02: attB4-CAGp>-attB5

   Plasmid

   #162972

   Purpose

   Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   chicken beta-actin/CMV enhancer promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. C453-E24: attB4-UbCp>-attB5

   Plasmid

   #162980

   Purpose

   Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human Ubiquitin C promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C512-E23: attB5r-mRuby3-nostop-attB1r

   Plasmid

   #162945

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Ruby3 fluorescent protein
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C512-E22: attB5r-mClover3-nostop-attB1r

   Plasmid

   #162944

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Clover3 fluorescent protein
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. C238-E02: attB2r-BGHpolyA-attB3

   Plasmid

   #162916

   Purpose

   Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Bovine growth hormone polyadenylation sequence
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMaCTag-18

   Plasmid

   #119997

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mKeima8.5
   

   Use

   Pcr template

   Tags

   mKeima8.5

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-ERas-IH

   Plasmid

   #15688

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   ERas (Eras Mouse)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pMXs-EGFP-Rheb SSVM-IP

   Plasmid

   #13832

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

   Insert

   Rheb SSVM (Rheb Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C181 changed to serine

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pFUS_B8

    Plasmid

    #31025

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUS_B9

    Plasmid

    #31026

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG75-β2AR-T4L-sfGFP

    Plasmid

    #102461

    Purpose

    Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 104455

    Insert

    β2AR
    

    Tags

    His tag and sfGFP

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SNAP-EF(N)-30nmSAH-GFP(1-10)

    Plasmid

    #61662

    Purpose

    30nm SAH scaffold with EF Hand and split GFP attachment sites

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    SNAP-EF(N)-30nmSAH-GFP(1-10)
    

    Expression

    Mammalian

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cry2PHR - IBAR

    Plasmid

    #176602

    Purpose

    Cry2PHR with C-terminal IBAR domain

    Depositor

    Robert Hughes

    Article

    Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

    Insert

    Cry2PHR-mCherry-IBAR
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaCTag-01

    Plasmid

    #119980

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxBFP
    

    Use

    Pcr template

    Tags

    moxBFP

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS426-GFP-Num1-PH

    Plasmid

    #71374

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Num1-PH
    

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa2563-2692

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

17. GGE021

    Plasmid

    #120778

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T2-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACEBac1-NEDD1

    Plasmid

    #178073

    Purpose

    Insect cell expression of NEDD1

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    NEDD1 (NEDD1 Human)
    

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUS_B7

    Plasmid

    #31024

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. TOPO Venus

    Plasmid

    #68493

    Purpose

    TOPO construct expressing Venus for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    split Venus
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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