We narrowed to 6,802 results for: &alpha

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD4 Flag

Plasmid

#74896

Purpose

Express COMMD4 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD4 (COMMD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pROF402

Plasmid

#155334

Purpose

Binary vector for expression of FLuc controlled by the blue light-repressible system

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF441

Plasmid

#155336

Purpose

Binary vector for expression of a gRNA targeting AtAP1 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD7 Flag

Plasmid

#74899

Purpose

Express COMMD7 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD7 (COMMD7 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLVE-FH-IN

Plasmid

#70071

Purpose

2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-PP1A

Plasmid

#128348

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP1A (PPP1CA Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD6 Flag

Plasmid

#74898

Purpose

Express COMMD6 in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD6 (COMMD6 Human)

Tags

Flag

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-α1L9'S-nAchR

Plasmid

#20684

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrna1 Mouse)

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pSicoR-Ef1a-mCh-GFPi

Plasmid

#31849

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pFETCh_GATAD2A

Plasmid

#86279

Purpose

Donor vector for 3' FLAG tag of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD2A homology arms (GATAD2A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB321

Plasmid

#68649

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-Luci

Plasmid

#31850

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

Luciferase RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB121

Plasmid

#68575

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pProEX-Af2Sir2

Plasmid

#8380

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Tags

His

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
PET11a-Af2sir2

Plasmid

#8383

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Expression

Bacterial

Mutation

PET11a-Af2Sir2D103N

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pCaSpeR4 cyto Grx1-roGFP2

Plasmid

#64999

Purpose

Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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AtS/MAR10 insulator sequence (GB3458)

Plasmid

#160638

Purpose

pEGB3a1 AtS/MAR10

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

AtS/MAR10 insulator sequence

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPuroR-MCS-GAL4-MiniWhite

Plasmid

#165890

Purpose

Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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