We narrowed to 14,826 results for: NTS

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pJG493

Plasmid

#91197

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gUbi1+gUbi8+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAT9993-Hypa-ABE

Plasmid

#163001

Purpose

plasmid expressing Hypa-ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9676-ABE

Plasmid

#162997

Purpose

plasmid expressing ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CBM9-ID-F

Plasmid

#171639

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-ID-H1

Plasmid

#171709

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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cpc-PHLS (g)

Plasmid

#52311

Purpose

Replaces the cpc operon in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTaCas9_5

Plasmid

#91168

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-H840A_2

Plasmid

#91163

Purpose

Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A (nickase)

Use

CRISPR

Expression

Plant

Mutation

H840A

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00486

Plasmid

#39591

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5965 (woc Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pJG486

Plasmid

#91195

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi1+donor

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2 Krt4 Src (YF)-uniRapR-cerulean

Plasmid

#45509

Depositor

Klaus Hahn, Anna Huttenlocher

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src (YF)-uniRapR-cerulean

Use

Zebrafish expression

Tags

cerulean

Mutation

Y732F

Promoter

krt4

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSP64R1 SHP2 WT

Plasmid

#8325

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pMOD_C3106

Plasmid

#91101

Purpose

Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13 Aphrodite

Plasmid

#13570

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19-NLS-4

Plasmid

#54222

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 NLS (PCNA Human)

Tags

SV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSP64R1 SHP2 D61A

Plasmid

#8326

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Xenopus expression

Mutation

D61A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pDGE673

Plasmid

#153239

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-1

Plasmid

#22711

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 1 (mir-1 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMOD_A0701

Plasmid

#91019

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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