167,601 results

1. MBP-FUS_FL_WT

   Plasmid

   #98651

   Purpose

   Expresses MBP-tagged full length FUS

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi 41BBL-CD86 mIL-15

   Plasmid

   #183245

   Purpose

   Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNO286-3

   Plasmid

   #107746

   Purpose

   Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

   Depositor

   Jeffrey Tabor

   Article

   Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

   Inserts

   CcaS
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Deleted AA 223 and 512

   Promoter

   J23106 and J23108

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsPatL1_I

   Plasmid

   #146547

   Purpose

   Mammalian Expression of HsPatL1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsPatL1 (PATL1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVD442

   Plasmid

   #11074

   Depositor

   Michael Donnenberg, James Kaper

   Article

   Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

   Insert

   sacB (sacB Bacillus subtilis)
   

   Expression

   Bacterial

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ104 - Pmyo-3::mCherry::unc-54

   Plasmid

   #19328

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-3::mCherry
   

   Tags

   mCherry

   Expression

   Worm

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis-SNAP-PI(4,5)P2 Probe

   Plasmid

   #211513

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   PLCdelta1-PH
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

   Plasmid

   #64613

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   S33A, S37A, T41A, S45A

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-LgBit

   Plasmid

   #234738

   Purpose

   Mammalian expression of large NanoBit fragment (LgBit)

   Depositor

   Nevin Lambert

   Article

   Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

   Insert

   LgBit
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX229

    Plasmid

    #23002

    Depositor

    Linda Wordeman

    Article

    Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

    Insert

    Kif18A (KIF18A Human)
    

    Tags

    6His and EGFP

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-PH-FAPP1

    Plasmid

    #161986

    Purpose

    Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

    Insert

    PH-FAPP1 (PLEKHA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Wnt10A

    Plasmid

    #35920

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt10A (WNT10A Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-L1PA1-CH-mneo

    Plasmid

    #51288

    Purpose

    contains the fully codon optimized ORF1 and ORF2 of the human L1RP

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 (L1RE1 Human)
    

    Tags

    mneoI indicator cassette containing an inverted n…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBV-Luc

    Plasmid

    #16539

    Purpose

    luciferase reporter plasmid with very low basal activity

    Depositor

    Bert Vogelstein

    Article

    He et al Cell. 1999 Oct 29. 99(3):335-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET_RTX_(exo-)

    Plasmid

    #102786

    Purpose

    Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase with 3'-5' deficient exonuclease
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT375

    Plasmid

    #125616

    Purpose

    Expresses evoAPOBEC1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoAPOBEC1-BE4max-NG
    

    Expression

    Mammalian

    Mutation

    E4K H109N H122L D124N R154H A165S P201S F205S

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid

    #202037

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Insert

    hM4Di
    

    Use

    AAV

    Tags

    dTomato

    Promoter

    CAG

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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