We narrowed to 19,051 results for: Tec;

1. pRSETa-Amrose v3

   Plasmid

   #79539

   Purpose

   pRSETa (Invitrogen) plasmid expressing Amrose variant 3

   Depositor

   Randolph Caldwell

   Article

   Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

   Insert

   Amrose v3
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIII-Neo-E2Crimson

   Plasmid

   #153425

   Purpose

   Retroviral (MSCV) expression of E2-Crimson fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   E2-Crimson
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK233 (AAVS1 CMV-AtAFB2)

   Plasmid

   #140537

   Purpose

   AAVS1 CMV-AtAFB2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   CMV-AtAFB2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB3047(gRNA XII-1)

   Plasmid

   #73289

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 CTTN

   Plasmid

   #67100

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CTTN (CTTN Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pTS2353_Tier3(PB)-YPet_PuroR

   Plasmid

   #169652

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL2295

   Plasmid

   #36728

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   APC-TALEN_Right (APC Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pJL1-TTlight-4xDQNAT

   Plasmid

   #128394

   Purpose

   In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBICI_2_strong

   Plasmid

   #155168

   Purpose

   see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-MBP-(CBX1)3-eReader

    Plasmid

    #135090

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered x3
    

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

    Plasmid

    #191177

    Purpose

    Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:GAPDH.s1:EGFP (frameshifted)
    

    Expression

    Mammalian

    Mutation

    EGFP is frameshifted

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-tRNA-Ser

    Plasmid

    #174318

    Purpose

    For in vitro transcription of mouse tRNAs

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    tRNA-ser
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 GTF2B

    Plasmid

    #67067

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2B (GTF2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. TAL2255

    Plasmid

    #36698

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCC-TALEN_Right (Fancc Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. UASGAL-CU2-Pleum

    Plasmid

    #60328

    Purpose

    Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pPK1E-1+t

    Plasmid

    #48147

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 IFNG

    Plasmid

    #67056

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IFNG (IFNG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)

    Plasmid

    #52906

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[2]+Venus
    

    Use

    Retroviral

    Tags

    L-IFP-F[2]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid

    #52907

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[1]+Plum
    

    Use

    Retroviral

    Tags

    L-IFP-F[1]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

20. EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)

    Plasmid

    #191157

    Purpose

    Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp long

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP2:Bdnf.s1:mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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