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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,418 results for: 158

Showing: 9481 - 9510 of 10418 results

  1. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

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  3. pT/CAGGS-mVenus-P2A-NRASG12V

    Plasmid
    #236072
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Tags
    Expression
    Mutation
    G12V
    Promoter
    CAGGS
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236072-plasmid-map-sequence-id-472895

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  4. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid
    #111850
    Purpose
    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12V (KRAS Human)
    Use
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Valine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111850-plasmid-map-sequence-id-213464

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  13. pTripz NEO DEST

    Plasmid
    #209072
    Purpose
    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector to be used…
    Tags
    3X FLAG
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209072-plasmid-map-sequence-id-412301

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  24. pInducer10b-HA-KRAS WT CO SR

    Plasmid
    #164926
    Purpose
    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence
    Depositor
    Ji Luo
    Article
    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.
    Insert
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mutation
    9 Valine codons are optimized and siRNA targetin…
    Promoter
    Ubc promoter
    Available since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    164926-plasmid-map-sequence-id-333718

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  25. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid
    #210513
    Purpose
    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Inserts
    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and TetO
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210513-plasmid-map-sequence-id-415575

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  26. pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156662
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    KIR2DL4 (KIR2DL4 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156662-plasmid-map-sequence-id-430454

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  29. pINDUCER20_mStrawberry_Atg4BC74A

    Plasmid
    #161735
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mStrawberry
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161735-plasmid-map-sequence-id-320115

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Showing: 9481 - 9510 of 10418 results