We narrowed to 18,318 results for: Mut

1. pcDNA3.2-R5A-K6A C11orf83-V5

   Plasmid

   #65850

   Purpose

   Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Mutated UQCC3 (UQCC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R5A-K6A

   Promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.2-R5D-K6D C11orf83-V5

   Plasmid

   #65851

   Purpose

   Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Mutated UQCC3 (UQCC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R5D-K6D

   Promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

   Plasmid

   #65660

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   changed glutamic acid 97 to alanine, lysine 100 t…

   Promoter

   CMV, 2x Tet

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-N1-hRIP1-FL I541P

   Plasmid

   #61381

   Purpose

   Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

   Depositor

   Francis Chan

   Article

   Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

   Insert

   Full length human RIP1with I541P mutation (RIPK1 Human)
   

   Use

   Tags

   EGFP and HA tag

   Expression

   Mammalian

   Mutation

   changed I541 to P

   Promoter

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pSP64-hERG1a D540K

   Plasmid

   #53057

   Purpose

   Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Aspartate 540 to Lysine

   Promoter

   SP6

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABEpuro-ARAF_D429A

   Plasmid

   #51080

   Purpose

   mammalian expression of ARAF D429A

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D429A

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMM2-hPMCA4bct120

   Plasmid

   #47760

   Purpose

   mammalian expression of human PMCA4 with C-terminal 120aa deletion

   Depositor

   John Penniston, Emanuel Strehler

   Article

   Enyedi et al J Biol Chem. 1993 May 15;268(14):10621-6

   Insert

   PMCA4bct120 (ATP2B4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of C-terminal 120 aa (removes calmodulin…

   Promoter

   SV40

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pRK5-Myc-SH3BP4 W92A

   Plasmid

   #46892

   Purpose

   expresses Myc tagged SH3BP4 W92A mutant in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

   Insert

   SH3BP4 W92A (SH3BP4 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   W92A (mutated SH3 domain)

   Promoter

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET-MRPL15

   Plasmid

   #31345

   Depositor

   Linda Spremulli

   Article

   Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

   Insert

   mitochondrial ribosomal protein L15 mature form (MRPL15 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Has the amino acids in the predicted mature form …

   Promoter

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pET30-GBF-E6

    Plasmid

    #21696

    Depositor

    James Baleja

    Article

    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.

    Insert

    protein G domain B1 fused human papillomavirus type 16 amno acid residue 2-142 (E6 Human papillomavirus type 16)
    

    Use

    Tags

    Protein G B1 domain

    Expression

    Bacterial

    Mutation

    GB1 (1-56aa) fusion tag with mutations at three p…

    Promoter

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. UAS-u(XS)Cas9

    Plasmid

    #127382

    Purpose

    Expresses Cas9 at very high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TBG-mTSG

    Pooled Library

    #113585

    Purpose

    AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes in mouse liver

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Expression

    Mammalian

    Use

    AAV, CRISPR, Lentiviral, and Luciferase

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

13. EFS-mTSG

    Pooled Library

    #113584

    Purpose

    AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Expression

    Mammalian

    Use

    AAV, CRISPR, Lentiviral, and Luciferase

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pMYH9-WT-V5

    Plasmid

    #183512

    Purpose

    Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-TO-oNGN2

    Plasmid

    #198397

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

    Promoter

    CAG, EF1a, and TRE3G

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_ADAR2DD_E488Q

    Plasmid

    #205598

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    ADAR2 deaminase domain (ADARB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutation

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_ADAR2DD_E488Q_Ago2

    Plasmid

    #205599

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    hAgo2 (AGO2 Human)
    

    Use

    Tags

    ADAR2DD_E488Q (hyperactive mutant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. peGFP C3-SPG20-PAAA

    Plasmid

    #218581

    Purpose

    Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Proline 172 to Alanine and Tyrosine 174 t…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge