We narrowed to 7,432 results for: 261

1. pcs2-cDELE1(142-515)-GFP-IRES-mCherry

   Plasmid

   #226102

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   only encodes for residues 142-515 of DELE1 corres…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF0413

   Plasmid

   #142617

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CTCFL (CTCFL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF0410

   Plasmid

   #142616

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CTCFL (CTCFL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneo-lambdaN-HA-MBP_F

   Plasmid

   #146261

   Purpose

   Mammalian Expression of MBP

   Depositor

   Elisa Izaurralde

   Insert

   MBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAK_Tol2_TRE_Blast

   Plasmid

   #130261

   Purpose

   Tol2 doxycycline inducible expression vector

   Depositor

   Michael Yaffe

   Type

   Empty backbone

   Use

   Tol2 transposon, doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWPT-nlsLacZ

   Plasmid

   #12261

   Depositor

   Didier Trono

   Insert

   LacZ
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-FLAG-Nv-IkB

   Plasmid

   #27261

   Depositor

   Thomas Gilmore

   Insert

   Nv-IkB
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   P88Q

   Promoter

   Available since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF0338

   Plasmid

   #144261

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MECOM (MECOM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AP1muA-TurboID-polyA-G418 HR

    Plasmid

    #227734

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT/T0-Flag-BRIP1

    Plasmid

    #222619

    Purpose

    Mammalian expression vector of flag-tagged BRIP1

    Depositor

    Daniel Durocher

    Article

    Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

    Insert

    BRIP1 (BRIP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR223 SARS-CoV-2 NSP7

    Plasmid

    #141261

    Purpose

    Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid

    #199261

    Purpose

    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-2xFLAG-2xSTREP-CDK2

    Plasmid

    #172616

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-SLC38A1_STOP

    Plasmid

    #161261

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A1 (SLC38A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA3.1-2xFLAG-2xSTREP-CDK4

    Plasmid

    #172615

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_Ckm_F9

    Plasmid

    #226140

    Purpose

    AAV construct for HITI insert with Ckm gRNA and hF9 transgene

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Inserts

    F9 (F9 Human)
    Ckm gRNA (Ckm Mouse)
    EGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pD649-HAsp-TNFSF10-COMP5AP-AviTag-9xHis

    Plasmid

    #157261

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TNFSF10 (TNFSF10 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_HA.NWS.C_NGFR

    Plasmid

    #158261

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.NWS.C

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

    Plasmid

    #72610

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33 to alanine

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

    Plasmid

    #72613

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Mutation

    Changed serine37 to alanine

    Promoter

    TRE tight promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MINK1 gRNA (BRDN0001145021)

    Plasmid

    #76261

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MINK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MINK1 (MINK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid

    #72611

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33, threonine34 and serine37 to ala…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pLenti X2 Blast/shp16 (w112-1)

    Plasmid

    #22261

    Depositor

    Eric Campeau

    Insert

    sh cyclin-dependent kinase inhibitor 2A
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOP5GP

    Plasmid

    #112610

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKP968

    Plasmid

    #167653

    Purpose

    Express colicinB-His6, D17C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 D17C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with D17C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOP5GT

    Plasmid

    #112611

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOP5MP

    Plasmid

    #112612

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-MBP and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKP996

    Plasmid

    #167654

    Purpose

    Express colicinB-His6, T18C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 T18C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with T18C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKP995

    Plasmid

    #167655

    Purpose

    Express colicinB-His6, M19C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 M19C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with M19C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKP997

    Plasmid

    #167656

    Purpose

    Express colicinB-His6, V20C, M261C

    Depositor

    Kathleen Postle

    Insert

    cba-his6 V20C, M261C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba-his6 with V20C, M261C mutations

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOP5TT

    Plasmid

    #112613

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-thioredoxin and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT2-shP53

    Plasmid

    #124261

    Purpose

    Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shP53
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. SARS CoV-2 RBD (bio-His)

    Plasmid

    #166857

    Purpose

    Human expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.

    Depositor

    Evelien Bunnik

    Article

    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

    Insert

    SARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    6xHis and Biotinylation site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EGFP-INPP5E-CAAX

    Plasmid

    #202746

    Purpose

    express plasma membrane targeted fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid

    #166856

    Purpose

    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.

    Depositor

    Evelien Bunnik

    Article

    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

    Insert

    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    8xHis and Biotinylation site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

    Plasmid

    #162618

    Purpose

    Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M337V

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

    Plasmid

    #172618

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr283Ala

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge