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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,967 results for: his

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Showing: 621 - 640 of 22967 results

  8. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  11. pET15bHis6-pG-Tnp

    Plasmid
    #124884
    Purpose
    To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.
    Depositor
    Keji Zhao
    Insert
    protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124884-plasmid-map-sequence-id-243752

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  12. pET15bHis6-pG-MNase

    Plasmid
    #124886
    Purpose
    To transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.
    Depositor
    Keji Zhao
    Insert
    protein G-MNase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    124886-plasmid-map-sequence-id-243685

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  13. pML104-HIS3-3

    Plasmid
    #232882
    Purpose
    Plasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232882-plasmid-map-sequence-id-462144

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  14. pML104-HIS3-2

    Plasmid
    #232881
    Purpose
    Plasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    HIS3 gRNA (HIS3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    snR52
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232881-plasmid-map-sequence-id-462138

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  19. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid
    #226247
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226247-plasmid-map-sequence-id-450057

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Showing: 621 - 640 of 22967 results