We narrowed to 16,596 results for: puromycin

1. pAAVS1-P-CAG-mCh

   Plasmid

   #80492

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   mCherry
   

   Use

   Donor vector (human)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-HA-MRE11-H129N

   Plasmid

   #82034

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Nuclease-dead mutant: H129N MRE11

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM60 FLAG-RAB35 Q67L

   Plasmid

   #107105

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE-HA-MRE11-H63D

   Plasmid

   #82036

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Exonuclease-dead mutant: H63D MRE11

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiPGK Puro DEST ERKKTRClover

   Plasmid

   #90227

   Purpose

   Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIP hIL2 co

   Plasmid

   #90513

   Purpose

   Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   IL2 (IL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICE-HA-MRE11-H63S

   Plasmid

   #82035

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H63S MRE11 mutant

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGMC00029 (aka pRC0610)

   Plasmid

   #200834

   Purpose

   Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

    Plasmid

    #232183

    Purpose

    Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6, hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-sgRNA-A

    Plasmid

    #149369

    Purpose

    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CBh, PGK, and U6

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183606

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Promoter

    Mouse Pgk1

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-NLS-mCherry

    Plasmid

    #60364

    Purpose

    Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    NLS-mCherry
    

    Tags

    NLS from SV40 T antigen and mCherry

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-cGAS-N-HA

    Plasmid

    #130913

    Purpose

    Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS N terminus
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HSC1-HS4-GiP

    Plasmid

    #58540

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Human EF1-alpha

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscALPS-HIV-1 Group P-vpr

    Plasmid

    #115837

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group P Vpr (HQ179987)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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