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Dimer-FRB

Plasmid

#239847

Purpose

Expresses a de novo dimer fused to FRB

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

Dimer-FRB

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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3mer-FKBP

Plasmid

#239848

Purpose

Expresses a de novo 3mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

3mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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4mer-FKBP

Plasmid

#239849

Purpose

Expresses a de novo 4mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

4mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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6mer-FKBP

Plasmid

#239851

Purpose

Expresses a de novo 6mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

6mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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12mer-FKBP

Plasmid

#239852

Purpose

Expresses a de novo 12mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

12mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEZYpci

Plasmid

#18707

Purpose

pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Expression

Yeast

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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MAP80A_MS2

Plasmid

#220624

Purpose

tRNA reporter vector

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTEF1_MS2

Plasmid

#220625

Purpose

Source for TEF1 MS2 coat fusion protein fusion

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_SFV_E1E2

Plasmid

#207308

Purpose

Mammalian expression of Semliki Forest virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SFV_E1E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LgBiT-ST

Plasmid

#207633

Purpose

A plasmid encoding LgBiT fused to SpyTag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

LgBiT-SpyTag

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MAP72_MS2

Plasmid

#220623

Purpose

Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Use

Shuttle vector

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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PhoCoil

Plasmid

#220444

Purpose

A plasmid encoding a photodegradable hydrogel-forming protein PhoCoil

Depositor

Cole DeForest

Article

Gregorio et al Sci Adv. 2025 Aug 29;11(35):eadx3472. do

Insert

PhoCoil

Tags

6x His (N and C term)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3B07

Plasmid

#71040

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CoRest (CoRest Fly)

Use

Gateway Cloning

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
TEV Protease-mCherry

Plasmid

#243793

Purpose

Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TEV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSC-SmBiT

Plasmid

#207632

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiT

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-SmBiT

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_91_positive_Control

Plasmid

#130572

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC3

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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HRV-3C Protease-mCherry

Plasmid

#243792

Purpose

Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HRV-3C protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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HER2Nb-C AND (A OR T)-SpyTag

Plasmid

#243790

Purpose

Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HER2 nanobody

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_24_positive_Control

Plasmid

#130570

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC1

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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