165,185 results

1. pSpyB-GFP

   Plasmid

   #220190

   Purpose

   sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG35 promoter with GFP in sgRNA site
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG35

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXS-IRES-Blast FLVCR1b-3X FLAG

   Plasmid

   #218536

   Purpose

   human FLVCR1b with C-terminal 3X_FLAG cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Use

   Retroviral

   Tags

   FLAG (3X)

   Expression

   Mammalian

   Mutation

   Alternative isoform

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

   Plasmid

   #218534

   Purpose

   human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Use

   Retroviral

   Tags

   FLAG (3X)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast FLVCR2

   Plasmid

   #218537

   Purpose

   human FLVCR2 cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   FLVCR2 (FLVCR2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

   Plasmid

   #220075

   Purpose

   expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5 H478/9A (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H478/9A, deltaTMD

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

   Plasmid

   #220074

   Purpose

   expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5deltaORDdeltaTMD (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaORDdeltaTMD

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-C1-ORP5deltaTMD-FKBP

   Plasmid

   #220073

   Purpose

   expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5deltaTMD (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaTMD

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast SLC5A7

   Plasmid

   #218539

   Purpose

   human SLC5A7 cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SLC5A7 (SLC5A7 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast SLC44A1

   Plasmid

   #218538

   Purpose

   human SLC44A1 cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SLC44A1 (SLC44A1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-Blast FLVCR1b

    Plasmid

    #218535

    Purpose

    human FLVCR1b cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Alternative isoform

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-Blast FLVCR1/FLVCR1a

    Plasmid

    #218533

    Purpose

    human FLVCR1/FLVCR1a cDNA

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    FLVCR1 (FLVCR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQFT-mNG-Pcon-Scar

    Plasmid

    #203678

    Purpose

    pQFT-mNG with constitutively expressed mScarlet-I

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PQJ and Phyb18

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQFT-mNGsacB

    Plasmid

    #203676

    Purpose

    pQFT with mNeonGreen and sacB under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Inserts

    mNeonGreen
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Has synonymous (silent) mutations to remove BsrGI…

    Promoter

    PQJ

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL20_mEGFP_HEY1_NCOA2

    Plasmid

    #205829

    Purpose

    Express mEGFP-tagged fusion protein, HEY1_NCOA2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    HEY1_NCOA2 (HEY1, NCOA2 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC67897

    Plasmid

    #209491

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    HvU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC67899

    Plasmid

    #209493

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    HvU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EC70130

    Plasmid

    #209501

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC70190

    Plasmid

    #209451

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    HvU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Cas12i2-T2A-EGFP

    Plasmid

    #188270

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. QUAS-0-T7-GFP_ColE1

    Plasmid

    #171649

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-0-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. T7-LacO-QF_p15A

    Plasmid

    #171651

    Purpose

    Expresses QF activator protein in BL21(DE3) E. coli. Contains p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QF-T7 Stop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. QUAS-5-T7-GFP_ColE1

    Plasmid

    #171653

    Purpose

    QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-5-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. QUAS-10-T7-GFP_ColE1

    Plasmid

    #171654

    Purpose

    QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-10-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. QUAS-15-T7-GFP_ColE1

    Plasmid

    #171655

    Purpose

    QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-15-T7
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQCXIH-tag RFPt- Rab11a WT

    Plasmid

    #161992

    Purpose

    Retroviral expression vector containing Rab11a tagged with RFP

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    RAB11A (RAB11A Canis Lupus)
    

    Use

    Retroviral

    Tags

    RFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-CMV-Galpha-q ONE-GO

    Plasmid

    #189738

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-q ONE-GO
    GNAQ (Gnaq Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207860

    Purpose

    AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG-CFP

    Plasmid

    #11179

    Purpose

    Mammalian expression vector for expression of CFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    ECFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mKO2-N1

    Plasmid

    #54625

    Purpose

    Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

    Depositor

    Atsushi Miyawaki, Michael Davidson

    Article

    Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. rAAV2/MG1.1

    Plasmid

    #184540

    Purpose

    AAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsid

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

    Insert

    AAV-MG1.1 capsid
    

    Use

    AAV; Helper vector for packaging

    Tags

    Expression

    Mutation

    changes on AAV9 capsid: K449R, A587L, Q588M; inse…

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge