We narrowed to 19,003 results for: Tec

1. pCellFree_G03 CTNNB1

   Plasmid

   #67119

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. AV4-EF1-GFP

   Plasmid

   #124971

   Purpose

   Control for AV4-EF1-NMNAT2

   Depositor

   Kazuhiro Oka

   Article

   Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

   Insert

   GFP
   

   Use

   AAV

   Promoter

   EF1-alpha

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPK1E-2+t

   Plasmid

   #48148

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSTAP-seq_fly-zfh1

   Plasmid

   #86381

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer
   

   Use

   Stap-seq screening vector

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8e(TadA-8e V82G)

   Plasmid

   #138494

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e V82G)-nSpCas9
   

   Expression

   Mammalian

   Mutation

   TadA-8e V82G

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST-HipO

   Plasmid

   #114599

   Purpose

   Strong constitutive expression of metabolic enzyme, HipO, for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   HipO
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Promoter

   OR2-OR1-Pr

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL3060

   Plasmid

   #41234

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-dazl-Left (dazl Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

8. TAL3061

   Plasmid

   #41235

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-dazl-Right (dazl Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

9. TAL2364

   Plasmid

   #36777

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC1-TALEN_Left (Hdac1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TAL2383

    Plasmid

    #36792

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    AKT2-TALEN_Right (Akt2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. RFN_EGFP_47

    Plasmid

    #60071

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. drd4a_L (TAL 3006)

    Plasmid

    #35998

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting drd4a (drd4a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC215

    Plasmid

    #118879

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    

    Tags

    Dendra2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry_LD 0_CS M_tTA-BFP

    Plasmid

    #58874

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAL3030

    Plasmid

    #41204

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-bbc3-(puma)-Left (bbc3 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

16. mCherry_LD 0_CS A_tTA-BFP

    Plasmid

    #58873

    Purpose

    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOCC207

    Plasmid

    #118869

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mCherry

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAPcrRNA_Eco

    Plasmid

    #106276

    Purpose

    E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEcoCascade-IL1RNcrRNA

    Plasmid

    #126481

    Purpose

    Encodes E.coli CRISPR Type I-E crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Eco IL1RN cr26
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

    Plasmid

    #58830

    Purpose

    Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2 x Osh2 PH domain (OSH2 Budding Yeast)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Lysine 307 to Glutamate and Arginine 309 …

    Promoter

    PHO5

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only