249,296 results

1. pFB1.HMBP.PrS.PHF1

   Plasmid

   #125167

   Purpose

   Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF1 (PHF1 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1.HMBP.PrS.PHF1

   Plasmid

   #125167

   Purpose

   Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   PHF1 (PHF1 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pReg-RamR

   Plasmid

   #190610

   Purpose

   Expresses the RamR transcription factor in E. coli

   Depositor

   Andrew Ellington

   Article

   d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

   Insert

   RamR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

   Plasmid

   #164457

   Purpose

   AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SlugCas9

   Plasmid

   #163793

   Purpose

   Expresses SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sec61b-V5-TurboID

   Plasmid

   #166971

   Purpose

   Expresses ER targeted TurboID for secretome biotinylation

   Depositor

   Hyun-Woo Rhee

   Article

   Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

   Insert

   Sec61b-V5-TurboID
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEVY-GU

   Plasmid

   #51229

   Purpose

   bi-directional expression vector for yeast, galactose inducible, URA3 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1,10

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEVY-GU

   Plasmid

   #51229

   Purpose

   bi-directional expression vector for yeast, galactose inducible, URA3 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1,10

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Red cGull

   Plasmid

   #191853

   Purpose

   Expresses biosensor for cGMP in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

   Insert

   Red cGull
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO mScarlet (AAV5)

    Viral Prep

    #131002-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Promoter

    EF1a

    Tags

    mScarlet (Cre-dependent)

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

11. NPY-GFP

    Plasmid

    #74629

    Purpose

    human neuropeptide Y fused to GFP

    Depositor

    Justin Taraska

    Insert

    Neuropeptide Y (NPY Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. NPY-GFP

    Plasmid

    #74629

    Purpose

    human neuropeptide Y fused to GFP

    Depositor

    Justin Taraska

    Insert

    Neuropeptide Y (NPY Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF1α-Cre-2A-mScarlet

    Plasmid

    #207745

    Purpose

    Expression of the Cre recombinase linked to a mScarlet fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mScarlet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. qTAG-N-Blast-3xFLAG

    Plasmid

    #207684

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLVX-IRES-Gβγ-BERKY3

    Plasmid

    #158426

    Purpose

    BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Use

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nup133_mEGFP(-8a)

    Plasmid

    #163417

    Purpose

    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)

    Depositor

    Sanford Simon

    Insert

    Nup133 (NUP133 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSerOTS-C1* (V70)

    Plasmid

    #188537

    Purpose

    Phosphoserine incorporation into recombinant proteins

    Depositor

    Jesse Rinehart

    Article

    Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

    Inserts

    pSerRS
    Ef-pSer
    tRNApSer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations resulting from laboratory evolution

    Promoter

    glnS*

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF3431

    Plasmid

    #144907

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR3C2 (NR3C2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGWB415

    Plasmid

    #74809

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGWB415

    Plasmid

    #74809

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. kiCAP-AAV-MyoAAV1A

    Plasmid

    #224437

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDLTTP insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TREtight-mTagBFP2-B19G

    Plasmid

    #100799

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-TREtight-mTagBFP2-B19G

    Plasmid

    #100799

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL4-ERSE1-luc2P-Hygro

    Plasmid

    #101789

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-I
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge