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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,887 results for: Coli

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Showing: 6501 - 6520 of 9887 results

  1. pfMDH_D230A

    Plasmid
    #67725
    Purpose
    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed aspartate 230 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pfMDH_N191A

    Plasmid
    #67724
    Purpose
    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 191 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pfMDH_K295A

    Plasmid
    #67726
    Purpose
    Expresses K295A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 295 to alanine
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pHL 1454

    Plasmid
    #53019
    Purpose
    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Promoter
    pLlac0-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid
    #82528
    Purpose
    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    amino acids 1-84 with mutation of RKR70-72, K75, …
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82528-plasmid-map-sequence-id-156824

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  13. pHL 1916

    Plasmid
    #53023
    Purpose
    AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    Asp terminator and RBS(st7)
    Expression
    Bacterial
    Promoter
    pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pHL 1011

    Plasmid
    #52993
    Purpose
    pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    RBS (st7)
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Promoter
    pLlac0-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL1756

    Plasmid
    #53036
    Purpose
    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Promoter
    pConNoHind and pConNoHindM12
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pSTTa

    Plasmid
    #248647
    Purpose
    E. coli protein expression vector encoding 2x N-terminal StrepII tags flanking an 11 residue flexible linker, thrombin and TEV protease sites, and an optional C-terminal FLAG tag.
    Depositor
    Sarah Assmann
    Article
    Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.
    Type
    Empty backbone
    Tags
    2x StrepII, Thrombin site, TEV site and FLAG
    Expression
    Bacterial
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    248647-plasmid-map-sequence-id-497014

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Showing: 6501 - 6520 of 9887 results