We narrowed to 103,955 results for: TRI

1. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

   Plasmid

   #83946

   Purpose

   Cas9-carrying vector with kanMX marker

   Depositor

   Irina Borodina

   Article

   Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNG1299

   Plasmid

   #149709

   Purpose

   Template for Malachite Green Aptamer (MGA) transcription assay

   Depositor

   Peter Lewis

   Article

   Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

   Insert

   Triple Promoter - 12X MG Aptamer Array - Terminator construct
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-CAG-Tfe3-pgk-hph

   Plasmid

   #48755

   Purpose

   PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Insert

   Tfe3 (Tcfe3 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. 6xHis(TEV)-Drp1-StrepII

   Plasmid

   #174422

   Purpose

   Expresses human Drp1 Isoform 3 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-2xMreBN-mCherry-His

   Plasmid

   #133626

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   2xMreB-mCherry-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mVenus-hCOL1A1

   Plasmid

   #140111

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK015

   Plasmid

   #105347

   Purpose

   Bacterial translocation fo proteins into plant cells

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   secretion signal, ccdB cassette
   

   Use

   Bacterial secretion

   Tags

   Expression

   Mutation

   BsaI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mApple-hCOL1A1

   Plasmid

   #140113

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK013

   Plasmid

   #105345

   Purpose

   Bacterial translocation fo proteins into plant cells

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   secretion signal, ccdB cassette
   

   Use

   Bacterial secretion

   Tags

   Expression

   Mutation

   BsaI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCK016

    Plasmid

    #105348

    Purpose

    Bacterial translocation for proteins into plant cells

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    secretion signal, ccdB cassette
    

    Use

    Bacterial secretion

    Tags

    Expression

    Mutation

    BsaI restriction sites removed

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK014

    Plasmid

    #105346

    Purpose

    Bacterial translocation fo proteins into plant cells

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    secretion signal, ccdB cassette
    

    Use

    Bacterial secretion

    Tags

    Expression

    Mutation

    BsaI restriction sites removed

    Promoter

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCl-MCS_miniCMV_miRFP703

    Plasmid

    #134985

    Purpose

    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    miRFP703
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5-ALK2 Q207D

    Plasmid

    #11740

    Depositor

    Jeff Wrana

    Article

    Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

    Insert

    ALK2 (ACVR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q207D: activated receptor

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pCPP5741

    Plasmid

    #128722

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcV-hopV1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-JNKAR1

    Plasmid

    #61625

    Purpose

    FRET-based biosensor for monitoring JNK activity

    Depositor

    Jin Zhang

    Article

    Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    JNKAR1
    

    Use

    Tags

    Citrine and ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPP5910

    Plasmid

    #128717

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcO-hopO1-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCPP5058

    Plasmid

    #128710

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcF-hopF2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-mFzd7

    Plasmid

    #42259

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 7 (Fzd7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

19. GFP-TMCC3

    Plasmid

    #121048

    Purpose

    Expresses human TMCC3 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Insert

    Trans membrane and coiled coil domain containing protein 3 (TMCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. cr3Vn

    Plasmid

    #184861

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Use

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRK5-mWnt3a

    Plasmid

    #42277

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Wnt3a (Wnt3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pSCB2-sgRNA

    Plasmid

    #129463

    Purpose

    Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    pgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pE478G-EGFP

    Plasmid

    #68848

    Purpose

    mutated OPTN was inserted into pEGFP-N1 backbone

    Depositor

    Beatrice Yue

    Article

    Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E478G, associated with ALS

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP mCherry-ApoB-EGFP

    Plasmid

    #112859

    Purpose

    Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

    Depositor

    Silvestro Conticello

    Article

    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

    Insert

    mCherry-apoB-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-TMCC2

    Plasmid

    #121047

    Purpose

    Expresses human TMCC2 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Insert

    Trans membrane and coiled coil domain containing protein 2 (TMCC2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5 HA3-eDHFR-T2A-puro

    Plasmid

    #86395

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A puro
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A puro

    Expression

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Promoter

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CMV-CAR-GECO1

    Plasmid

    #45493

    Purpose

    Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    CAR-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PclpB riboswitch GFP pZa

    Plasmid

    #170078

    Purpose

    GFP expression under the control of E. coli clpB promoter engineered with riboregulators

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PclpB

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 6xHis(TEV)-Drp1(K38A)-StrepII

    Plasmid

    #174423

    Purpose

    Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K38A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCB301TOR-CRISPR

    Plasmid

    #164797

    Purpose

    For gene editing in oomycetes

    Depositor

    Miaoying Tian

    Article

    Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

    Inserts

    PsNLS-hSpCas9
    sgRNA expression cassette
    

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    HAM34 and RPL41

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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