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We narrowed to 16,712 results for: grna

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Showing: 6521 - 6540 of 16712 results

  2. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  3. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse TTN for activation
    Expression
    Mammalian
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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  4. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Expression
    Mammalian
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

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  5. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human TTN for activation
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128323-plasmid-map-sequence-id-255024

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  6. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

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  7. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    EFS
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. LentiCRISPR_Hs_MBD3_ex3

    Plasmid
    #62624
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    EFS
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  10. pCJ1022

    Plasmid
    #228762
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Pkd2 gRNAs (Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    228762-plasmid-map-sequence-id-455850

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  11. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV Retrograde)

    Viral Prep
    #125712-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Promoter
    short version of αCamKinase II promoter (0.4kb)
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only

  13. pCB32

    Plasmid
    #111911
    Purpose
    Expresses gRNA to target Nte1 locus and has HygR marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting Nte1 locus
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    SNR52p
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111911-plasmid-map-sequence-id-218313

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  14. pCB30

    Plasmid
    #111910
    Purpose
    Expresses gRNA to target URA3 knockout locus and has KanMX marker
    Depositor
    Yi Tang
    Insert
    gRNA cassette targeting URA3 knockout locus
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    SNR52p
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    111910-plasmid-map-sequence-id-218310

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  16. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  17. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Plasmids for introduction of genes into 11 predetermined yeast chromosomal loci using CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6521 - 6540 of 16712 results