We narrowed to 17,881 results for: puromycin
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Plasmid#129412PurposeEncoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 and sgACSL3.C (ACSL3 Human)
UseCRISPRTagsP2A-PuroExpressionMammalianPromoterhU6Available sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-CUL4A WT-P2A-Puro
Plasmid#124304PurposeLentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoterDepositorAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
library backbone 3'LTR gRNA and barcode
Plasmid#127397PurposeDigestion by BstXI and BamHI to release the backboneDepositorTypeEmpty backboneUseLentiviralTagsPuro-mCherryAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN1-C911
Plasmid#125784Purposea seed-matched control for pRSITEP-puro-shWRN1DepositorInsertshWRN1-C911 (WRN Human)
UseRNAiAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-puro-shWRN2
Plasmid#125782Purposeconstitutive expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN2 (WRN Human)
UseRNAiAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-G185R
Plasmid#125849Purposeexpress mutant BAP1-G185RDepositorAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgSTAT3-1
Plasmid#121425PurposesgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgSTAT3-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…MutationSilent V205V mutation in myogenin gene to prevent…Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2601_eSpCas9-G2P
Plasmid#124203PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneExpressionMammalianAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-1
Plasmid#92156PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-1
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shID2 #1
Plasmid#83086PurposeLentiviral shRNA vector for inducible knockdown of human ID2DepositorAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1413 GFP-TDP43 VLIM-F
Plasmid#118802PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll VLIM to F in CTD of full-length TDP43Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX307 HRAS
Plasmid#117748PurposeOpen reading frame vector encoding HRASDepositorInsertRASH1 (HRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationClosed vector so will not read through the V5 reg…PromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B CPM2
Plasmid#121163PurposeMammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZFP36L1 (TIS11B) charge pattern mutant 2
ExpressionMammalianAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TIS11B CPM2
Plasmid#121143PurposeMammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)
ExpressionMammalianAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MTOR HEAT
Plasmid#120709PurposeExpresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus productionDepositorInsertMTOR HEAT 1-9 (MTOR Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 1-409PromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro
Plasmid#120564PurposeExpresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationQ61KPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MPHOSPH8 ts2
Plasmid#115875PurposeMPHOSPH8 knockdownDepositorAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts1
Plasmid#115871PurposeFAM208a knockdownDepositorAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by