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pJ2042.2

Plasmid

#140670

Purpose

3-color stripe

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pICH50932

Plasmid

#48050

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pICH50872

Plasmid

#48044

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pTHSSe_2

Plasmid

#109234

Purpose

Empty SpecR p15A vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Type

Empty backbone

Expression

Bacterial

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-Top2A-5X Gly-Venus-T2A-Hygro

Plasmid

#211115

Purpose

For tagging the C-term of Top2A with Venus

Depositor

Job Dekker

Publication

Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

Inserts

5' C-term Top2A homology arm (TOP2A Human)
Venus
T2A
Blastocidin Resistance
5' C-term Top2A homology arm (TOP2A Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

n/a

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWBs02

Plasmid

#222478

Purpose

Gateway vector for 35s promoter-driven expression in Marchantia polymorpha carrying a sulfadiazine selection marker.

Depositor

Philip Carella

Publication

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-C-MBP

Plasmid

#214304

Purpose

Provide a bacterial expression vector that encodes C-terminus MBP tag

Depositor

Cliff Stains

Publication

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Type

Empty backbone

Expression

Bacterial

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Plac-SO

Plasmid

#218358

Purpose

Empty vector with lacIq-Plac expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet1

Plasmid

#218360

Purpose

Empty backbone with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJ2077.2

Plasmid

#140666

Purpose

Controller plasmid for TS circuit and cL and cR controls

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Controller plasmid for TS circuit and cL and cR controls

Use

Synthetic Biology

Expression

Bacterial

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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EB2316

Plasmid

#87753

Purpose

pkaiBC::EYFP cloned between XbaI and XhoI of EB2065

Depositor

Erin O'Shea

Publication

Zheng et al Cell Rep. 2017 Apr 18;19(3):497-504. doi

Insert

pkaiBC::EYFP

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSEVA621-AzFRS

Plasmid

#242002

Purpose

Expressed AzF tRNA synthetase for incorporation of p-azido-L-phenylalanine (pAzF)

Depositor

Wei Niu

Publication

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

AzFRS

Expression

Bacterial

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1

Plasmid

#202429

Purpose

Mammalian expression vector containing mNeonGreen

Depositor

David Bryant

Publication

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Type

Empty backbone

Use

Lentiviral

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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actc1b-GcAMP5

Plasmid

#126563

Purpose

Muscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APT

Depositor

Rob Parton

Publication

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

GcAMP5

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Publication

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Promoter

T7

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pNC1053

Plasmid

#20406

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP

Expression

Yeast

Mutation

linker: delta k

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pTG612

Plasmid

#237387

Purpose

mStayGold^NSEC^ repair template

Depositor

Ariel Pani

Publication

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold^NSEC^

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-EV

Plasmid

#202466

Purpose

Empty vector of pSMART-HCKan

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pKanB

Plasmid

#223523

Purpose

Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Publication

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMP117

Plasmid

#207920

Purpose

GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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