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pMGT1

Plasmid

#109404

Purpose

Tetracycline resistance reporter of ChnR activation

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

Tetracycline resistance reporter of ChnR activation

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx6

Plasmid

#74345

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 6 (Dlx6 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-LEU2

Plasmid

#65334

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

LEU2

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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ERroGFP_C147S_pCDNA3

Plasmid

#48630

Purpose

Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFP with Cysteine 147 to Serine mutation (roGFP C147S, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERroGFP

Tags

FLAG

Expression

Mammalian

Mutation

C147S

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-prk:nptII

Plasmid

#239580

Purpose

Selection plasmid for Rubisco directed evolution

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PRK-NPTII

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn2

Plasmid

#206963

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pH1-shControl-pSyn-tdtomato

Plasmid

#206319

Purpose

Control for pH1-shOPA1-pSyn-tdtomato

Depositor

Yusuke Hirabayashi

Article

Suga et al PLoS Biol. 2023 Aug 31;21(8):e3002246. d

Insert

shRNA scramble control

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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WT-β3

Plasmid

#166579

Purpose

For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

β3 (Itgb3 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-KanMx

Plasmid

#122923

Purpose

TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-NatMx

Plasmid

#122924

Purpose

TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HIS3

Plasmid

#122912

Purpose

TRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-LEU2

Plasmid

#122905

Purpose

ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

Plasmid

#106182

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

hSynapsin

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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POT4-TDH3p-ycrtI-ADH1t

Plasmid

#65355

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFAB815

Plasmid

#47854

Purpose

BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rnpB T1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO9

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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ERrefGFP1_pCDNA3

Plasmid

#48635

Purpose

Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERrefGFP1

Tags

FLAG

Expression

Mammalian

Mutation

C147S,C204Q

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pLV.CMVenh.gp91.GP91.cHS4

Plasmid

#30479

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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