We narrowed to 9,772 results for: Tec;

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GFP-ERK2 del174-177

Plasmid

#118297

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Article

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 del174-177 (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deletion 174aa-177aa

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGBT9-hSyntenin2b

Plasmid

#89422

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Tags

GAL4 DBD

Expression

Yeast

Mutation

* see below

Promoter

ADH1

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Article

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSOD1E133K-AcGFP1

Plasmid

#83442

Purpose

Expresses SOD1E133K in mammalian cells.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

SOD1 (SOD1 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

E133K

Promoter

CMV

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 neo

Plasmid

#72569

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL3

Plasmid

#67418

Purpose

Expression of hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL6-no stop

Plasmid

#67329

Purpose

hArl6 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL8A-no stop

Plasmid

#67333

Purpose

hArl8a without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF5

Plasmid

#67312

Purpose

hArf5 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 477 T to C; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL2-no stop

Plasmid

#67319

Purpose

hArl2 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
NET4 pEGFP-N2 (1201)

Plasmid

#62041

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

Net4 (TMEM53 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) HIPK3 Antisense

Plasmid

#60635

Purpose

Negative Control for the pcDNA3.1(+) HIPK3 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Homeodomain-interacting protein kinase 3 (HIPK3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM3-K83A

Plasmid

#58401

Purpose

Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24A

Plasmid

#58400

Purpose

Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lysine 24 to Alanine

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC

Plasmid

#47364

Purpose

Bmal fragment cloned with Venus tag and used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDNR-hSox2

Plasmid

#49389

Purpose

donor vector for cloning human Sox2 with 6xHN tag

Depositor

Randall McKinnon

Insert

Sox2 (SOX2 Human)

Use

Cre/Lox; Donor vector

Tags

6xHN (6 repeating His-Asn subunits)

Mutation

TGA (stop) to CCA (Pro) at nt 1027 with respect t…

Promoter

none

Available Since

Nov. 20, 2013

Availability

Academic Institutions and Nonprofits only