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pLL3.7m-psMEK1tight

Plasmid

#89362

Purpose

A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

Depositor

Michael Lin

Publication

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1tight (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-MYT1L

Plasmid

#32926

Purpose

Retroviral expression of human Myt1L

Depositor

Kevin Eggan

Publication

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

MYT1L (MYT1L Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMXs-Ngn2

Plasmid

#32928

Purpose

Retroviral expression of mouse Ngn2

Depositor

Kevin Eggan

Publication

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Ngn2 (Neurog2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDEST17-UbE2G2

Plasmid

#15791

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEcCas-2.0

Plasmid

#190697

Purpose

Constitutive expressing of Cas9 and inducible expression of lambda Red

Depositor

Sheng Yang

Publication

Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

Insert

Cas9 and Lamda Red system

Expression

Bacterial

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Publication

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

Plasmid

#97044

Purpose

Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pStart-T2

Plasmid

#20347

Depositor

Mario Capecchi

Publication

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Type

Empty backbone

Use

Mouse Targeting

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Publication

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-EGFP(HiBiT)

Plasmid

#162589

Purpose

N-terminally HiBiT-tagged EGFP under CAG promoter

Depositor

Masaharu Somiya

Publication

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Tags

HiBiT

Expression

Mammalian

Promoter

CAG promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mFGF8b

Plasmid

#22090

Depositor

Gail Martin

Publication

Crossley et al Development. 1995 Feb . 121(2):439-51.

Insert

FGF8 (Fgf8 Mouse)

Use

Bacterial

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Lenti-TetON-Sox2

Plasmid

#110280

Purpose

Doxycycline-inducible overexpression of Sox2

Depositor

Trudy Oliver

Publication

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

TRE

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEcCas-2.0

Plasmid

#190697

Purpose

Constitutive expressing of Cas9 and inducible expression of lambda Red

Depositor

Sheng Yang

Publication

Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

Insert

Cas9 and Lamda Red system

Expression

Bacterial

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Publication

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-IFP2.0 T2A HO1

Plasmid

#59427

Purpose

Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

Depositor

Xiaokun Shu

Publication

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Insert

IFP2.0T2AHO1 IRES EGFP

Use

Lentiviral

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pStart-T2

Plasmid

#20347

Depositor

Mario Capecchi

Publication

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Type

Empty backbone

Use

Mouse Targeting

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-EGFP(HiBiT)

Plasmid

#162589

Purpose

N-terminally HiBiT-tagged EGFP under CAG promoter

Depositor

Masaharu Somiya

Publication

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Tags

HiBiT

Expression

Mammalian

Promoter

CAG promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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