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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,348 results for: multi

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Showing: 6561 - 6580 of 18348 results

  2. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Publication
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  3. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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  4. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid
    #115487
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115487-plasmid-map-sequence-id-240326

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  8. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid
    #98975
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 4 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98975-plasmid-map-sequence-id-199218

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  9. px330 VRER

    Plasmid
    #101729
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101729-plasmid-map-sequence-id-265859

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  10. EKARdual

    Plasmid
    #99870
    Purpose
    Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Marc Tramier
    Publication
    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EKAR
    Tags
    ShadowG and mTFP1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    99870-plasmid-map-sequence-id-182629

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  13. pET15b LFn-Fla 3A

    Plasmid
    #84872
    Purpose
    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Publication
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Tags
    His
    Expression
    Bacterial
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    T7
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  14. pCru5-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49226
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    Viral LTR (or CMV)
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    49226-plasmid-map-sequence-id-73641

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Showing: 6561 - 6580 of 18348 results