We narrowed to 7,375 results for: ef1a

1. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

   Plasmid

   #193752

   Purpose

   human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC374 - pAAV EF1a DIO iRFP

   Plasmid

   #47626

   Purpose

   An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO-EGFP-WPRE-pA

   Plasmid

   #37084

   Purpose

   Cre-dependent expression of EGFP

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox; Cre-on

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVH-EF1a-GFP-P2A-OCT4

   Plasmid

   #130692

   Purpose

   Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-Ronin-FLAG-IRES-Neo

   Plasmid

   #28020

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Insert

   Ronin (THAP11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO-oG-WPRE-hGH

   Plasmid

   #74290

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   Ef1a

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-N2c_envA-IRES-Neo

   Plasmid

   #172368

   Purpose

   Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   envA + Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

   Plasmid

   #170229

   Purpose

   Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Q64L

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-PG13-mCherry-EF1a-PuroR-EGFP

   Plasmid

   #195292

   Purpose

   piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

   Depositor

   Hideyuki Oguro

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   mCherry
   PuroR-P2A-EGFP
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

    Plasmid

    #203844

    Purpose

    Flp-dependent Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-tdTomato-WPRE-pGHpA

    Plasmid

    #67527

    Purpose

    "Expresses the fluorescent protein tdTomato"

    Depositor

    Botond Roska

    Article

    Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

    Insert

    tandem dimer tomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

    Plasmid

    #171027

    Purpose

    Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1 and AAV9

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    ChrimsonR is fused to tdTomato

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVH-EF1a-GFP-P2A-SOX2

    Plasmid

    #130693

    Purpose

    Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0045-EF1a-PguCas13b-NES-HIV

    Plasmid

    #103861

    Purpose

    Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PguCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-UbC-FKRP-EF1a-BSD

    Plasmid

    #205150

    Purpose

    Express UbC-driven FKRP and EF-1a driven BSD

    Depositor

    Monkol Lek

    Insert

    Fukutin-related protein (FKRP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

    Plasmid

    #201032

    Purpose

    Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

    Plasmid

    #100941

    Purpose

    Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
    

    Use

    Tags

    1xHA and 3xTy1

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

    Plasmid

    #189807

    Purpose

    Retroviral delivery of Cas9

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAC150-PBLHL-4xHS-EF1a-DEST

    Plasmid

    #48234

    Purpose

    PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #133786

    Purpose

    Cre-dependent tdTomato fluorescent reporter for cell labeling

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    pAAV-EF1a-DIO-tdTomato-WPRE
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAF0690 Ef1a-Kp03_attB-BFP acceptor

    Plasmid

    #193473

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-Ef1a-ConVERGD-eGFP-W3SL

    Plasmid

    #218744

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1a-DIO-ASAP3-WPRE

    Plasmid

    #132318

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lv EF1a SS18-Frb PGK Puro

    Plasmid

    #102811

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    SS18-Frb
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid

    #35498

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-EF1a-hDNAJC6-T2A-copGFP

    Plasmid

    #170443

    Purpose

    Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human DNAJC6 (DNAJC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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