We narrowed to 1,889 results for: IRES-GFP

1. pLV UBC APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #214983

   Purpose

   Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   APEX2 (APX2 Glycine max (soybean))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   UBC

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

   Plasmid

   #58959

   Purpose

   lentiviral expression of v-H-Ras and luciferase/EGFP reporter

   Depositor

   Snorri Thorgeirsson

   Article

   Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

   Inserts

   CMV enhancer/chicken beta actin promoter
   v-H-Ras
   luc2/EGFP fusion gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer/chicken beta actin

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

   Plasmid

   #134869

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome b5 type A (CYB5A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-98

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-IRES-GFP/CALR del52-FLAG

   Plasmid

   #204523

   Purpose

   Retroviral expression of human CALR del52-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR del52-FLAG (CALR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   del52

   Promoter

   Available since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. UbC-TRE-mSOD2-tTS-IRES-EGFP

   Plasmid

   #12390

   Depositor

   Zuoshang Xu

   Article

   Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

   Insert

   mSOD2 shRNA (Sod2 Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102885

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-GFP-SFPQ-IRES-mCherry

   Plasmid

   #166950

   Purpose

   Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   GFP and mCherry

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CAG-rtTA-IRES-eGFP-pGK-NeoR

   Plasmid

   #204497

   Purpose

   Expresses rtTA and Neomycin resistance genes in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Insert

   rtTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL CAG NFlag BAF170 ires GFP

   Plasmid

   #24562

   Depositor

   Jerry Crabtree

   Article

   Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

   Insert

   BAF170 (Smarcc2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pMSCV-IRES-GFP/CALR ins5-FLAG

    Plasmid

    #204524

    Purpose

    Retroviral expression of human CALR ins5-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR ins5-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    ins5

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and hSyn

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid

    #32522

    Depositor

    Mustafa Sahin

    Insert

    dominant negative Rheb (D60K) (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D60K to make dominant negative

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

14. MSCV-BRD9-PGK-Puro-IRES-GFP

    Plasmid

    #75114

    Purpose

    Retroviral expression plasmid encoding wild type human BRD9

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

    Plasmid

    #208695

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG

    Plasmid

    #81023

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

17. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-Gluc-RMA-IRES-EGFP

    Plasmid

    #189628

    Purpose

    Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and TRE

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #81022

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. MSCV-mouse Ezh2 Y637S-IRES-GFP

    Plasmid

    #91887

    Purpose

    Retroviral expression of mouse Ezh2 with Y637S

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Y637S

    Promoter

    MMLV gag

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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