We narrowed to 22,419 results for: LEA

1. Myopathy Loop Mutant Nuclear Myosin I YFP

   Plasmid

   #60243

   Purpose

   Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   Deleted I339, I340, G343, E344, E345,L346

   Promoter

   CMV

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75177

   Purpose

   Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Three prime entry vector for multisite gateway th…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

   Plasmid

   #105806

   Purpose

   Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-Cerulean; ORF2: TEV-Amber

   Expression

   Mammalian

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104618

   Purpose

   Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   hsp70-Syn21-FLAG-mCerulean-P10
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_HA-p115-TEV-cleaved #957#382

   Plasmid

   #42107

   Depositor

   Ayano Satoh

   Article

   Koreishi et al Antibody Technology Journal. 2011;1:5-11

   Insert

   p115
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted 886 to end and added ENLYFQ

   Promoter

   CMV

   Available Since

   Feb. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLeAPS-GFP-blast

   Plasmid

   #182230

   Purpose

   Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   GFP-P2A-Blasticidin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCK054 pDEST exorh:mCerulean

   Plasmid

   #195985

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. nuclear NAD+ biosensor

   Plasmid

   #186789

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK002 pME mCerulean

   Plasmid

   #195956

   Purpose

   Gateway pME fluorophore

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. nuclear cpVenus control

    Plasmid

    #186790

    Purpose

    Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pATetO 6XHis T5 Exonuclease

    Plasmid

    #206209

    Purpose

    Inducible expression of T5 exonuclease

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    T5 exonuclease
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN001 pME minprom_mCerulean

    Plasmid

    #195959

    Purpose

    Gateway pME fluorophore

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter mCerulean
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available Since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mRNA-CleanCap-mNeonGreen

    Plasmid

    #188248

    Purpose

    Template plasmid to transcribe mRNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-nuclear-EKAR4

    Plasmid

    #174439

    Purpose

    Nuclear-targeted EKAR4.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    nuclear-EKAR4
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAF020 p3E-2A-mCerulean

    Plasmid

    #195974

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-mCerulean
    

    Use

    Other

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nuclear Myosin I YFP

    Plasmid

    #60241

    Purpose

    Expresses nuclear isoform of myosin IC attached to YFP and an NLS

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRNA-CleanCap-NanoLuc

    Plasmid

    #188247

    Purpose

    Template plasmid to transcribe mRNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL2-2xCLEAR-luciferase

    Plasmid

    #81120

    Purpose

    Reporter activity of the CLEAR network

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    2xCLEAR
    

    Use

    Luciferase

    Tags

    luciferase

    Promoter

    2xCLEAR

    Available Since

    Dec. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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