We narrowed to 12,317 results for: LEA
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Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227AAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sgCtr- LentiCRISPRv2
Plasmid#107402PurposeLentiviral expression of Cas9 and a control gRNADepositorInsertcontrol gRNA
UseLentiviralAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 TFAM-mClover
Plasmid#129574PurposeExpression of mClover fused TFAMDepositorAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorPublicationInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
TagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…Available sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKSI-1
Plasmid#51725Purposecloning backboneDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Thy1-Brainbow-1.1 M
Plasmid#18726DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Thy1-Brainbow-2.1 R
Plasmid#18727DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)
Viral prep#55645-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Csy4H29AS50C
Plasmid#45029DepositorInsertCsy4H29AS50C
TagsHis6-MBPExpressionBacterialMutationH29A mutation to abolish catalytic activity and S…PromoterT7Available sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialPromoterT7Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2 SENP2
Plasmid#13382DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSENP2 (SENP2 Human)
TagsEGFPExpressionMammalianAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT413
Plasmid#8606DepositorInsertbarnase, barstar
TagsphoA signal sequence to barnaseExpressionBacterialAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 luciferase
Plasmid#26612DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfirefly luciferase
ExpressionMammalianAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15b-His-RF2
Plasmid#124130PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)
Viral prep#112677-AAV5PurposeReady-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLUT7 HA-GLI1
Plasmid#62970PurposeExpresses HA-tagged Gli1DepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by